Document Detail

Reactive oxygen species trigger ischemic and pharmacological postconditioning: in vivo and in vitro characterization.
MedLine Citation:
PMID:  17915258     Owner:  NLM     Status:  MEDLINE    
Reactive oxygen species (ROS) generated by ischemic and pharmacological preconditioning are known to act as triggers of cardiac protection; however, the involvement of ROS in ischemic and pharmacological postconditioning (PostC) in vivo and in vitro is unknown. We tested the hypothesis that ROS are involved in PostC in the mouse heart in vivo and in the isolated adult cardiac myocyte (ACM). Mice were subjected to 30 min coronary artery occlusion followed by 2 h of reperfusion with or without ischemic or pharmacologic PostC (three cycles of 20 s reperfusion/ischemia; 1.4% isoflurane; 10 mg/kg SNC-121). Additional groups were treated with 2-mercaptopropionyl glycine (MPG), a ROS scavenger, 10 min before or after the PostC stimuli. Ischemia-, isoflurane-, and SNC-121- induced PostC reduced infarct size (24.1+/-3.2, 15.7+/-2.6, 24.9+/-2.6%, p<0.05, respectively) compared to the control group (43.4+/-3.3%). These cardiac protective effects were abolished by MPG when administered before (40.0+/-3.6, 39.3+/-3.1, 38.5+/-1.6%, respectively), but not after the PostC stimuli (26.6+/-2.3, 17.0+/-2.2, 23.9+/-1.7%, respectively). Additionally, ACM were subjected to a simulated ischemia/reperfusion protocol with isoflurane and SNC PostC. Isoflurane- and SNC-induced PostC in vitro were abolished by prior treatment with MPG. These data indicate that ROS signaling is an essential trigger of ischemic and pharmacological PostC and this is occurring at the level of the cardiac myocyte.
Yasuo M Tsutsumi; Takaakira Yokoyama; Yousuke Horikawa; David M Roth; Hemal H Patel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-09-09
Journal Detail:
Title:  Life sciences     Volume:  81     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-10-09     Completed Date:  2007-12-06     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1223-7     Citation Subset:  IM    
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MeSH Terms
Analgesics, Opioid / administration & dosage,  pharmacology,  therapeutic use
Cells, Cultured
Free Radical Scavengers / pharmacology
Hemodynamics / drug effects
Ischemic Preconditioning, Myocardial*
Isoflurane / administration & dosage,  pharmacology,  therapeutic use
Mice, Inbred C57BL
Myocardial Infarction / metabolism,  physiopathology,  prevention & control*
Myocardial Reperfusion
Myocardial Reperfusion Injury / metabolism*,  physiopathology
Myocardium / metabolism*
Myocytes, Cardiac / drug effects,  metabolism*
Reactive Oxygen Species / metabolism*
Grant Support
Reg. No./Substance:
0/Analgesics, Opioid; 0/Free Radical Scavengers; 0/Reactive Oxygen Species; CYS9AKD70P/Isoflurane

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