| Reactive oxygen species production is increased in the peripheral blood monocytes of obese patients. | |
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MedLine Citation:
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PMID: 19439330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infiltrating macrophages play an important role in the production of inflammatory mediators by the adipose tissue of obese subjects. To reach the adipose tissue, peripheral monocytes are recruited by locally produced chemoattractants. However, little is known about the activation of monocytes in the peripheral blood of obese subjects. The objective of this study was to determine reactive oxygen species and endoplasmic reticulum stress as early markers of monocytic commitment with an inflammatory phenotype in the peripheral blood of nondiabetic obese patients. Patients were recruited from an academic general hospital; controls were voluntary students. Seven lean controls and 6 nondiabetic obese patients were included in the study. Monocytes were prepared from peripheral blood. Immunoblot, flow cytometry, and polymerase chain reaction were used to determine reactive oxygen species and endoplasmic reticulum stress. Increased reactive oxygen species and activation of endoplasmic reticulum stress were detected in the monocytes from obese patients. Reducing endoplasmic reticulum stress with a chemical chaperone reversed monocytic activation, as determined by the reduction of reactive oxygen species production. Thus, monocytes from nondiabetic obese patients are already committed with an inflammatory phenotype in peripheral blood; and reducing endoplasmic reticulum stress negatively modulates their activation. |
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Authors:
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Giovanna R Degasperi; Raphael G P Denis; Joseane Morari; Carina Solon; Bruno Geloneze; Christiane Stabe; José Carlos Pareja; Aníbal E Vercesi; Lício A Velloso |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-18 |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 58 ISSN: 1532-8600 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-20 Completed Date: 2009-08-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: United States |
Other Details:
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Languages: eng Pagination: 1087-95 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University of Campinas, Campinas-SP 13084-970, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Calcium / metabolism Catalase / metabolism Cytosol / metabolism DNA-Binding Proteins / metabolism Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum / metabolism* Female Flow Cytometry Humans Immunoblotting Immunoprecipitation Inflammation / blood, metabolism* Male Monocytes / enzymology, metabolism* Obesity / blood* Oxidative Stress* Phenotype Polymerase Chain Reaction RNA Splicing RNA, Messenger / metabolism Reactive Oxygen Species / blood* Superoxide Dismutase / metabolism Transcription Factors / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/RNA, Messenger; 0/Reactive Oxygen Species; 0/Transcription Factors; 0/regulatory factor X transcription factors; 7440-70-2/Calcium; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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