| Reactive oxygen species production by monocytes and polymorphonuclear leukocytes during dialysis. | |
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MedLine Citation:
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PMID: 1996568 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intradialytic production of reactive oxygen species (ROS) by monocytes and polymorphonuclear leukocytes (PMNL) was examined separately in six hemodialysis patients. Samples obtained 15 minutes after initiation of dialysis with new cuprophane membranes demonstrated significantly increased (P less than 0.05) ROS production in both cell populations as measured by the fluorescence of a specific intracellular marker (2',7'-dichlorofluorescein diacetate [DCFH-DA]) assayed by flow cytometry. Granulocytes harvested during dialysis also showed decreased responsiveness to exogenous C5a and F-Met-Leu-Phe (FMLP) at 15, 30, and 60 minutes after initiation of dialysis (P less than 0.05). Our data suggest that hemodialysis with cuprophane membrane is associated with monocyte and PMNL activation as shown by production of ROS coincident with peak activation of the complement cascade; these granulocytes become refractory to further stimulation with C5a and FMLP during dialysis. |
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Authors:
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J Himmelfarb; J M Lazarus; R Hakim |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of kidney diseases : the official journal of the National Kidney Foundation Volume: 17 ISSN: 0272-6386 ISO Abbreviation: Am. J. Kidney Dis. Publication Date: 1991 Mar |
Date Detail:
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Created Date: 1991-03-28 Completed Date: 1991-03-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8110075 Medline TA: Am J Kidney Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 271-6 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Vermont Medical School, Maine Medical Center, Portland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Cellulose / analogs & derivatives*, pharmacology Complement C5a Female Flow Cytometry Fluoresceins Humans Hydrogen Peroxide / metabolism* Lymphocyte Activation Male Membranes, Artificial Middle Aged Monocytes / immunology* N-Formylmethionine Leucyl-Phenylalanine / pharmacology Neutrophils / immunology* Renal Dialysis* |
| Grant Support | |
ID/Acronym/Agency:
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R01-HL36015/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Membranes, Artificial; 2044-85-1/diacetyldichlorofluorescein; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 7722-84-1/Hydrogen Peroxide; 80295-54-1/Complement C5a; 9004-34-6/Cellulose; 9050-09-3/cuprammonium cellulose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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