Document Detail

Reactive oxygen species production by monocytes and polymorphonuclear leukocytes during dialysis.
MedLine Citation:
PMID:  1996568     Owner:  NLM     Status:  MEDLINE    
Intradialytic production of reactive oxygen species (ROS) by monocytes and polymorphonuclear leukocytes (PMNL) was examined separately in six hemodialysis patients. Samples obtained 15 minutes after initiation of dialysis with new cuprophane membranes demonstrated significantly increased (P less than 0.05) ROS production in both cell populations as measured by the fluorescence of a specific intracellular marker (2',7'-dichlorofluorescein diacetate [DCFH-DA]) assayed by flow cytometry. Granulocytes harvested during dialysis also showed decreased responsiveness to exogenous C5a and F-Met-Leu-Phe (FMLP) at 15, 30, and 60 minutes after initiation of dialysis (P less than 0.05). Our data suggest that hemodialysis with cuprophane membrane is associated with monocyte and PMNL activation as shown by production of ROS coincident with peak activation of the complement cascade; these granulocytes become refractory to further stimulation with C5a and FMLP during dialysis.
J Himmelfarb; J M Lazarus; R Hakim
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of kidney diseases : the official journal of the National Kidney Foundation     Volume:  17     ISSN:  0272-6386     ISO Abbreviation:  Am. J. Kidney Dis.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-03-28     Completed Date:  1991-03-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8110075     Medline TA:  Am J Kidney Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  271-6     Citation Subset:  IM    
Department of Medicine, University of Vermont Medical School, Maine Medical Center, Portland.
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MeSH Terms
Cellulose / analogs & derivatives*,  pharmacology
Complement C5a
Flow Cytometry
Hydrogen Peroxide / metabolism*
Lymphocyte Activation
Membranes, Artificial
Middle Aged
Monocytes / immunology*
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Neutrophils / immunology*
Renal Dialysis*
Grant Support
Reg. No./Substance:
0/Fluoresceins; 0/Membranes, Artificial; 2044-85-1/diacetyldichlorofluorescein; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 7722-84-1/Hydrogen Peroxide; 80295-54-1/Complement C5a; 9004-34-6/Cellulose; 9050-09-3/cuprammonium cellulose

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