Document Detail

Reactive oxygen species inhibit hyposmotic stress-dependent volume regulation in cultured rat cardiomyocytes.
MedLine Citation:
PMID:  17045960     Owner:  NLM     Status:  MEDLINE    
Cells have developed compensatory mechanisms to restore cell volume, and the ability to resist osmotic swelling or shrinkage parallels their resistance to necrosis or apoptosis. There are several mechanisms by which cells adapt to hyposmotic stress including that of regulatory volume decrease. In ischemia and reperfusion, cardiomyocytes are exposed to hyposmotic stress, but little is known as to how their volume is controlled. Exposure of cultured neonatal rat cardiomyocytes to hyposmotic media induced a rapid swelling without any compensatory regulatory volume decrease. The hyposmotic stress increased the production of reactive oxygen species, mainly through NADPH oxidase. Adenoviral overexpression of catalase inhibited the hyposmosis-dependent OH(*) production, induced the regulatory volume decrease mechanism, and prevented cell death. These results suggest that hyposmotic stress of cardiomyocytes stimulates production of reactive oxygen species which are closely linked to volume regulation and cell death.
Jessica Díaz-Elizondo; Mario Chiong; Diego Rojas-Rivera; Claudio Olea-Azar; H Moo Kwon; Sergio Lavandero
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-09
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  350     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2006-12-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1076-81     Citation Subset:  IM    
Departamento Bioquímica y Biología Molecular, Universidad de Chile, Santiago 8380492, Chile.
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MeSH Terms
Animals, Newborn
Cell Size
Cells, Cultured
Myocytes, Cardiac / cytology*,  physiology*
Osmotic Pressure
Oxidative Stress / physiology
Reactive Oxygen Species / metabolism*
Water-Electrolyte Balance / physiology*
Reg. No./Substance:
0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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