Document Detail

Reactive oxygen species induced by beauvericin, patulin and zearalenone in CHO-K1 cells.
MedLine Citation:
PMID:  19596061     Owner:  NLM     Status:  MEDLINE    
The cytotoxic effects of mycotoxins, induction of reactive oxygen species (ROS) and generation of lipid peroxidation products in CHO-K1 cells were determined as function of increasing time of exposure and concentrations of beauvericin (BEA), patulin (PAT) and zearalenone (ZEA). The end points were evaluated after 24h of exposure, by the tetrazolium salt (MTT) and neutral red (NR) assays. The IC(50) values obtained on the MTT and NR assays ranged from 0.69 to 79.40 microM and 4.40 to 108.76 microM, respectively. To determine the intracellular production of ROS, the intensity of fluorescence emitted from the probe H(2)-DCFDA was measured. The relative intensity of fluorescence from cells incubated with BEA, PAT and ZEA was approximately 4-, 7- and 4-fold higher in comparison with control cells at 0 min, respectively. Lipid peroxidation induced by ROS generation was assessed using the thiobarbituric acid reactive substances (TBARS) method for 2, 24 and 48 h. The malondialdehyde (MDA) production was increased with BEA and PAT exposure in a concentration- and time-dependent manner. MDA was not increased after 1 and 5 microM ZEA exposures for 2h, but was slightly increased at 50 microM. In conclusion, PAT was the most cytotoxic mycotoxin to CHO-K1 cells, followed by BEA and ZEA. Mycotoxins reduce cell viability correlated with the increases of ROS generation and MDA formation in concentration- and time-dependent manner. These data suggested that cytotoxicity and ROS generation are mechanisms of mycotoxins mediated toxicity.
E Ferrer; A Juan-Garc??a; G Font; M J Ruiz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-23
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  23     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-13     Completed Date:  2010-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  1504-9     Citation Subset:  IM    
Laboratori de Toxicologia, Facultat de Farmacia, Universitat de Valencia, Av. Vicent Andres Estelles, 46100 Burjassot, Valencia, Spain.
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MeSH Terms
CHO Cells
Depsipeptides / pharmacology*
Lipid Peroxidation / drug effects
Malondialdehyde / metabolism
Mycotoxins / pharmacology*
Patulin / pharmacology*
Reactive Oxygen Species / metabolism*
Zearalenone / pharmacology*
Reg. No./Substance:
0/Depsipeptides; 0/Mycotoxins; 0/Reactive Oxygen Species; 149-29-1/Patulin; 17924-92-4/Zearalenone; 26048-05-5/beauvericin; 542-78-9/Malondialdehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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