| Reactive oxygen species, especially O2+* in cancer mechanisms. | |
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MedLine Citation:
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PMID: 20192122 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the nature including the human organism there are 5 reactive oxygen species (ROS): O2, O2-*, O2+*, 1O2, O3 with different electromagnetic characteristics and biological effects. The effects of enrichment of medical oxygen O2 with traces of ROS on various cells were tested in experiments. METHODS: Human embryonic lung fibroblasts WI38 damaged by Radon Rn222 into WI38/ Rn cells and WI38 cells transformed by virus SV40 into VA13 cells were exposed to different ROS gas mixtures, prepared in high - voltage plasma chamber of "Oxygen Ion 3000". Trans-membrane resting potential (TMRP) was measured and cells morphology was visually observed using microscopy during 10 days. RESULTS: Exposition of WI38, WI38/Rn and VA13 to medical O2, alone show no effect in TMRP and in cell morphology. But pico-concentration of O2+* in medical O2 increased the TMRP in WI38/Rn cells from -25mV to -35mV (variance 0.5-2mV) (p < 0.001) with improvement of cells morphology and the TMRP of VA13 cells from -15mV to -32mV (2-3mV) (p < 0.001) with a maximum effect on the 5th day. Later the TMRP strongly decreased and the cell membrane ruptured due to water influx. O2, enriched with O2-* alone or together with O2+* had no significant effect in WI38/Rn and VA13 groups (p > 0.05). CONCLUSION: Radon protective effect on WI38/Rn or destructive effect on VA13 of pico-concentrations of O2+* in medical O2 conforms to the theory of hormesis resp. to hypothesis of cancer induction mechanisms, supporting it's further experimental or clinical use. |
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Authors:
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Ivan Engler; Christian Atzm?eller; Viliam Donic; Friedrich Steinh?usler |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of experimental therapeutics & oncology Volume: 8 ISSN: 1359-4117 ISO Abbreviation: J. Exp. Ther. Oncol. Publication Date: 2009 |
Date Detail:
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Created Date: 2010-03-02 Completed Date: 2010-03-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9604933 Medline TA: J Exp Ther Oncol Country: United States |
Other Details:
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Languages: eng Pagination: 157-65 Citation Subset: IM |
Affiliation:
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Department of Physiology, University P. J. Safarik, Faculty of Medicine, Trieda SNP 1, 04001 Kosice, Slovakia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinogens, Environmental Cell Line, Tumor Cell Shape Cell Transformation, Neoplastic / chemistry Female Fibroblasts / drug effects, radiation effects* Humans Lung Neoplasms / pathology Membrane Potentials / drug effects Neoplasms / metabolism*, pathology Radon Reactive Oxygen Species / metabolism*, radiation effects Simian virus 40 / genetics |
| Chemical | |
Reg. No./Substance:
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0/Carcinogens, Environmental; 0/Reactive Oxygen Species; 10043-92-2/Radon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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