Document Detail

Reactive oxygen species, cell growth, and taxol production of Taxus cuspidata cells immobilized on polyurethane foam.
MedLine Citation:
PMID:  16377848     Owner:  NLM     Status:  MEDLINE    
Dynamic changes in reactive oxygen species (ROS) of Taxus cuspidata cells immobilized on polyurethane foam were investigated and the relation between ROS content and taxol production was discussed. Immobilization shortened the lag period of cell growth and moderately increased H2O2 and O2-* contents inside the microenvironment within the first 15 d. After 20 d, excessive production of H2O2 and O2-* was observed accompanied by marked increases in membrane lipid peroxidation and cell membrane permeability. The taxol content of immobilized cells was fourfold that of suspended cells at d 35. The addition of exogenous H2O2 barely affected malondialdehyde content and cell membrane permeability but led to an obvious accumulation of taxol. It is inferred that the intracellular and extracellular H2O2 inside the microenvironment might be one factor promoting taxol biosynthesis under the immobilization stress.
De-Ming Yin; Jin-Chuan Wu; Ying-Jin Yuan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  127     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-26     Completed Date:  2006-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  173-85     Citation Subset:  IM    
Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China.
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MeSH Terms
Cell Culture Techniques
Cells, Immobilized / metabolism
Paclitaxel / biosynthesis*
Reactive Oxygen Species / metabolism*
Taxus / cytology,  metabolism*
Reg. No./Substance:
0/Polyurethanes; 0/Reactive Oxygen Species; 33069-62-4/Paclitaxel

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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