| Reactive oxygen inducing vasoconstriction in the isolated perfused rat liver. | |
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MedLine Citation:
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PMID: 8026796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of reactive oxygen generation on intact livers was studied. Production of reactive oxygen species in perfused livers isolated from normal and endotoxin-treated rats was measured using chemically enhanced chemiluminescence. The resting state chemiluminescence of the livers increased on endotoxin administration and was maximal about 6 h after treatment. Chemiluminescence from the livers was further stimulated severalfold by inclusion of phorbol myristate acetate in the perfusion medium, reaching maximum intensity 3 h after endotoxin treatment. Oxygen consumption by the endotoxin-treated liver showed a transient increase followed by a significant decrease on phorbol myristate acetate stimulation, which was inhibited by dexamethasone. These results are consistent with the occurrence of a respiratory burst followed by oxygen-radical-species-induced vasoconstriction in the intact perfused liver. The evaluation of reactive oxygen species by resident and accumulated macrophages in the intact liver is made possible by these studies, and related effects on the liver could be conveniently and quantitatively followed using this model. |
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Authors:
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I Ikai; B Chance; C Kumar |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Free radical biology & medicine Volume: 16 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-08-05 Completed Date: 1994-08-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 539-45 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemiluminescent Measurements Dexamethasone / pharmacology Escherichia coli Free Radicals Lipopolysaccharides / pharmacology Liver / blood supply*, metabolism Male Oxygen Consumption Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism* Tetradecanoylphorbol Acetate / pharmacology Vasoconstriction / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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AA 07186/AA/NIAAA NIH HHS; HL 18708/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 0/Lipopolysaccharides; 0/Reactive Oxygen Species; 16561-29-8/Tetradecanoylphorbol Acetate; 50-02-2/Dexamethasone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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