| Reactive nitrogen and oxygen species, and iron sequestration contribute to macrophage-mediated control of Encephalitozoon cuniculi (Phylum Microsporidia) infection in vitro and in vivo. | |
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MedLine Citation:
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PMID: 20888426 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Encephalitozoon cuniculi (Phylum Microsporidia) infects a wide range of mammals, and replicates within resting macrophages. Activated macrophages, conversely, inhibit replication and destroy intracellular organisms. These studies were performed to assess mechanisms of innate immune responses expressed by macrophages to control E. cuniculi infection. Addition of reactive oxygen and nitrogen species inhibitors to activated murine peritoneal macrophages statistically significantly, rescued E. cuniculi infection ex vivo. Mice deficient in reactive oxygen species, reactive nitrogen species, or both survived ip inoculation of E. cuniculi, but carried significantly higher peritoneal parasite burdens than wild-type mice at 1 and 2 weeks post inoculation. Infected peritoneal macrophages could still be identified 4 weeks post inoculation in mice deficient in reactive nitrogen species. L-tryptophan supplementation of activated murine peritoneal macrophage cultures ex vivo failed to rescue microsporidia infection. Addition of ferric citrate to supplement iron, however, did significantly rescue E. cuniculi infection in activated macrophages and further increased parasite replication in non-activated macrophages over non-treated resting control macrophages. These results demonstrate the contribution of reactive oxygen and nitrogen species, as well as iron sequestration, to innate immune responses expressed by macrophages to control E. cuniculi infection. |
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Authors:
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Elizabeth S Didier; Lisa C Bowers; Aaron D Martin; Marcelo J Kuroda; Imtiaz A Khan; Peter J Didier |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-01 |
Journal Detail:
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Title: Microbes and infection / Institut Pasteur Volume: 12 ISSN: 1769-714X ISO Abbreviation: Microbes Infect. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-07 Completed Date: 2011-03-10 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 100883508 Medline TA: Microbes Infect Country: France |
Other Details:
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Languages: eng Pagination: 1244-51 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Institut Pasteur. Published by Elsevier SAS. All rights reserved. |
Affiliation:
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Division of Microbiology, Tulane National Primate Research Center, 18703 Three Rivers Road, Covington, LA 70433, USA. esdnda@tulane.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Encephalitozoon cuniculi / immunology* Encephalitozoonosis / immunology* Female Iron / metabolism* Macrophages, Peritoneal / immunology*, metabolism Mice Peritoneum / immunology, microbiology Reactive Nitrogen Species / metabolism*, toxicity Reactive Oxygen Species / metabolism*, toxicity Survival Analysis |
| Grant Support | |
ID/Acronym/Agency:
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AI039968/AI/NIAID NIH HHS; AI071778/AI/NIAID NIH HHS; P51 RR000164-488791/RR/NCRR NIH HHS; R01 AI039968-06/AI/NIAID NIH HHS; R01 AI071778-06/AI/NIAID NIH HHS; RR00164/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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