| Reactive change in proliferative activity of the junctional epithelium after topical application of lipopolysaccharide. | |
| | |
MedLine Citation:
|
PMID: 9203096 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
IT IS WELL ESTABLISHED THAT apical migration of junctional epithelium (JE) along a root surface is an important factor in periodontal pocket formation and deepening. However, the exact mechanism and, more specifically, the role of inflammatory products in influencing the activity of cells within the JE is not known. To address this issue lipopolysaccharide (LPS) was applied topically into rat molar gingival sulcus and then tissues evaluated immunohistochemically for expression of proliferating cell nuclear antigen (PCNA). Tissues were prepared for histological analysis at designated times. Histologically, infiltration of neutrophils with associated edema was noted in JE and gingival connective tissues 6 hours after LPS application and was prominent at 12 hours. These inflammatory changes persisted in the 2- and 3-day specimens, and disappeared at 5 days. In normal gingiva, before the LPS application, the JE showed few PCNA positive cells, while almost all cells in the basal and suprabasal cell layers of the oral gingival epithelium and the oral sulcular epithelium were PCNA positive. No increase in the number of PCNA positive cells in the JE beyond zero time was observed at 6 and 12 hours after LPS application. One day after LPS application, PCNA positive cells appeared in the basal cell layer of the JE, with a continued increase number of PCNA positive cells in the JE continued at 2 and 3 days. By day 5 the number of PCNA positive cells were decreasing with return to a normal range by 7 days. These results showed that 1) under normal physiological conditions, cells within the JE have minimal mitotic activity and 2) the JE cells can enter the proliferating cell cycle when exposed to LPS, and suggest that the enhanced proliferating activity in the JE is an important factor for the deepening of the periodontal pocket, if the connective tissue attachment is broken down. |
| | |
Authors:
|
T Takata; M Miyauchi; I Ogawa; H Ito; J Kobayashi; H Nikai |
Related Documents
:
|
3019256 - Dna polymerase activity in the n.hexadecane-induced hyperkeratotic epidermis. 2235986 - Infundibulocystic basal cell carcinoma: a newly described variant. 2463946 - Medial edge epithelium transforms to mesenchyme after embryonic palatal shelves fuse. 737716 - The unit structure of the locust compound eye. 17318626 - Regulation of pancreatic islet cell survival and replication by gamma-aminobutyric acid. 8644846 - Patterns of epidermal growth factor receptor amplification in malignant gliomas. |
Publication Detail:
|
Type: In Vitro; Journal Article |
Journal Detail:
|
Title: Journal of periodontology Volume: 68 ISSN: 0022-3492 ISO Abbreviation: J. Periodontol. Publication Date: 1997 Jun |
Date Detail:
|
Created Date: 1997-07-17 Completed Date: 1997-07-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 531-5 Citation Subset: D; IM |
Affiliation:
|
Department of Oral Pathology, Hiroshima University, School of Dentistry, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Division Epithelial Attachment / drug effects*, metabolism, pathology Escherichia coli Lipopolysaccharides / pharmacology* Male Periodontal Attachment Loss / etiology Periodontal Pocket / etiology Proliferating Cell Nuclear Antigen / biosynthesis Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
0/Lipopolysaccharides; 0/Proliferating Cell Nuclear Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Increased proliferation, collagen, and fibronectin production by hereditary gingival fibromatosis fi...
Next Document: Periodontal regeneration in naturally occurring Class II furcation defects in beagle dogs after guid...