| Reactive Oxygen Species (ROS) from NADPH and Xanthine Oxidase Modulate the Cutaneous Local Heating Response in Healthy Humans. | |
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MedLine Citation:
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PMID: 21436462 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Local cutaneous heating produces vasodilation that is largely nitric oxide (NO) dependent. We showed that Angiotensin II (Ang-II) attenuates this by an AT1R-dependent mechanism; is reversible with the antioxidant ascorbate, indicating oxidative stress. Reactive oxygen species (ROS) produced by Ang-II employ NADPH and xanthine oxidase pathways. To determine whether these mechanisms pertain to skin, we measured cutaneous local heating with 10 μM Ang-II, using Apocynin to inhibit NADPH oxidase and Allopurinol to inhibit xanthine oxidase. We also inhibited superoxide with Tempol, and H(2)O(2) with Ebselen. We heated the skin of the calf in 8 healthy volunteers (24.5-29.9 years) to 42°C and measured local blood flow to assess the percentage of maximum cutaneous vascular conductance. We re-measured while perfusing Allopurinol, Apocynin, Ebselen and Tempol through individual microdialysis catheters. This was then repeated with Ang-II combined with antioxidant drugs. Tempol and Apocynin alone had no effect on the heat response. Allopurinol enhanced the entire response (125% of heat-alone), while Ebselen suppressed the heat plateau (76% of heat-alone). Ang-II alone caused significant attenuation of the entire heat response (52%). When added to Ang-II, Allopurinol partially reversed the Ang-II attenuation. Heat with Ebselen and Angiotensin II were similar to heat and Ang-II; Ebselen only partially reversed the Ang-II attenuation. Apocynin and Tempol each partially reversed the attenuation caused by Ang-II. This suggests that ROS, produced by Ang-II via NADPH and xanthine oxidase pathways, modulates the response of skin to the application of heat, and thus contributes to the control of local cutaneous blood flow. |
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Authors:
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Marvin S Medow; Natasha Bamji; Debbie Clarke; Anthony J Ocon; Julian M Stewart |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-24 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1New York Medical College. |
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