Document Detail


Reactivation of sea-urchin sperm flagella induced by rapid photolysis of caged ATP.
MedLine Citation:
PMID:  9556534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sea-urchin sperm flagella in a state of rigor were reactivated by rapid photolysis of caged ATP. After a time lag of 11-17 ms, all bends in the axonemes present during rigor began to be propagated towards the tip as if their propagation had not been interrupted. This result suggests that the site-specific activity of dyneins along the length of the axoneme is preserved even during rigor states when ATP is absent and that regulation of the activity can be restarted immediately with a new cycle of ATP turnover. During the starting transient, pre-existing rigor waves in the distal region were propagated without a change in the maximal shear angle until they disappeared at the tip. This was more evident when the rapid reactivation was triggered in high-viscosity solution, in which only the form of new bends was greatly affected by viscous load. After reactivation, the velocity of microtubule sliding increased and reached a plateau within 28 ms. This time course reflects the rate of force generation by dynein in situ.
Authors:
T Tani; S Kamimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  201     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  1998 May 
Date Detail:
Created Date:  1999-01-06     Completed Date:  1999-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1493-503     Citation Subset:  IM    
Affiliation:
Department of Biology, Graduate College of Arts and Sciences, University of Tokyo, Tokyo, Japan. tani@nano.c.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analogs & derivatives*,  metabolism,  physiology
Animals
Flagella / physiology*
Male
Microtubules / metabolism,  physiology
Photolysis*
Sea Urchins
Spermatozoa / physiology*
Time Factors
Ultraviolet Rays
Viscosity
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 67030-27-7/P(3)-1-(2-nitro)phenylethyladenosine 5'-triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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