| Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. | |
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MedLine Citation:
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PMID: 16687171 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Auto-degradation of collagen matrices occurs in resin-infiltrated dentine by the slow action of host-derived matrix metalloproteinases. As phosphoric acid-etching inactivates these endogenous enzymes, it is puzzling how hybrid layers created by simplified etch-and-rinse adhesives can degrade in vivo. This study tested the null hypothesis that there are no differences in the relative proteolytic activities of mineralised dentine, acid-etched dentine, and etch-and-rinse adhesive-treated acid-etched dentine. Powdered dentine prepared from extracted human teeth was treated with 17% EDTA, 10% phosphoric acid, or with five simplified etch-and-rinse adhesives that were applied to 10% phosphoric acid-etched dentine. The gelatinolytic activity of the dentine powder was assayed using fluorescein-labelled gelatine. TEM examination of the air-dried, treated dentine powder was performed to confirm the presence of remnant mineralised dentine after acid-etching. 17% EDTA significantly reduced the relative proteolytic activity (73.2%) of the untreated mineralised dentine powder (control), while 10% phosphoric acid-etched dentine exhibited the highest reduction (98.1%). Treating the acid-etched dentine powder with any of the five simplified etch-and-rinse adhesives resulted in the reactivation of the proteolytic activity, with a significant negative linear correlation (P<0.05) between the increases in fluorescence and the corresponding pH values of the adhesives. It is concluded that simplified etch-and-rinse adhesives can reactivate endogenous enzymatic activities in dentine that are previously inactivated by phosphoric acid-etching. The amount of enzyme reactivated may even exceed the original quantity present in untreated mineralised dentine. This provides an explanation for the degradation of hybrid layers after acid-etched dentine matrices are infiltrated with these adhesives. |
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Authors:
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Annalisa Mazzoni; David H Pashley; Yoshihiro Nishitani; Lorenzo Breschi; Ferdinando Mannello; Leo Tjäderhane; Manuel Toledano; Edna L Pashley; Franklin R Tay |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-05-09 |
Journal Detail:
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Title: Biomaterials Volume: 27 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-05-22 Completed Date: 2006-08-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 4470-6 Citation Subset: IM |
Affiliation:
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Department of SAU and FAL, University of Bologna, Bologna, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Dentin
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chemistry* Hydrolysis Microscopy, Electron, Transmission Phosphoric Acids / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DE014911/DE/NIDCR NIH HHS; R01 DE015306/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphoric Acids; 7664-38-2/phosphoric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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