Document Detail


Reactivation of DNA synthesis in aging diploid human skin fibroblasts by fusion with mouse L karyoplasts cytoplasts and whole L cells.
MedLine Citation:
PMID:  7062789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diploid human skin fibroblasts derived from an 82-year-old donor with a 21-28 cell population doubling (CPD) range (where 28 CPD marked the end of the in vitro life span of the cells) were fused with whole L cells, L karyoplasts and L cytoplasts. The proportion of human nuclei incorporating tritiated thymidine after fusion was measured autoradiographically. Statistically significant increases in the labeling indices were found in the human nuclei in hybrid, heterodikaryon and cybrid cells when compared to control unfused human cells. Fusion of human diploid fibroblasts with human cytoplast derived from cells of the same CPD showed no significant changes in the labeling indices of the human nuclei.
Authors:
E G Nette; H L Sit; D W King
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  18     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1982 Jan 
Date Detail:
Created Date:  1982-05-12     Completed Date:  1982-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  75-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Aging
Animals
Cell Fusion
Cell Line
Cell Nucleus / metabolism
DNA / biosynthesis*
Diploidy
Fibroblasts / metabolism*
Humans
L Cells (Cell Line) / metabolism*
Mice
Mitotic Index
Skin / cytology
Thymidine / metabolism
Chemical
Reg. No./Substance:
50-89-5/Thymidine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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