Document Detail

Reactivation of DNA synthesis in aging diploid human skin fibroblasts by fusion with mouse L karyoplasts cytoplasts and whole L cells.
MedLine Citation:
PMID:  7062789     Owner:  NLM     Status:  MEDLINE    
Diploid human skin fibroblasts derived from an 82-year-old donor with a 21-28 cell population doubling (CPD) range (where 28 CPD marked the end of the in vitro life span of the cells) were fused with whole L cells, L karyoplasts and L cytoplasts. The proportion of human nuclei incorporating tritiated thymidine after fusion was measured autoradiographically. Statistically significant increases in the labeling indices were found in the human nuclei in hybrid, heterodikaryon and cybrid cells when compared to control unfused human cells. Fusion of human diploid fibroblasts with human cytoplast derived from cells of the same CPD showed no significant changes in the labeling indices of the human nuclei.
E G Nette; H L Sit; D W King
Related Documents :
10209749 - The assembly and migration of seqa-gfp fusion in living cells of escherichia coli.
6698029 - Inhibition of cell proliferation by interferons. 1. effects on cell division and dna sy...
16887819 - Analysis of cell migration, transdifferentiation and apoptosis during mouse secondary p...
11602749 - Antigenic properties of the human immunodeficiency virus envelope during cell-cell fusion.
10556979 - Differential expression of the il-1 system components during in vitro myogenesis: impli...
1527489 - Ploidy of bacillus subtilis exfusants: the haploid nature of cells forming colonies wit...
21648019 - Challenging the pluripotency of human testis-derived esc-like cells.
19723889 - Correcting mir-15a/16 genetic defect in new zealand black mouse model of cll enhances d...
15752719 - Reversible activation of secretory phospholipase a2 by sulfhydryl reagents.
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  18     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1982 Jan 
Date Detail:
Created Date:  1982-05-12     Completed Date:  1982-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  75-87     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Fusion
Cell Line
Cell Nucleus / metabolism
DNA / biosynthesis*
Fibroblasts / metabolism*
L Cells (Cell Line) / metabolism*
Mitotic Index
Skin / cytology
Thymidine / metabolism
Reg. No./Substance:
50-89-5/Thymidine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Age changes in human vocal muscle.
Next Document:  Influence of age on accumulation of alpha-actin in satellite-cell-derived myotubes in vitro.