Document Detail


Reaction-rate assay of serum sorbitol dehydrogenase activity of 37 degrees C.
MedLine Citation:
PMID:  240515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sorbitol dehydrogenase (EC 1.1.1.14) activity of human serum and liver was investigated at 37 degrees C. Various buffers were examined but tris(hydroxymethyl)aminomethane HCI in a final concentration of 90 mmol/liter (pH 6.6, at 37 degrees C) was the one with which activity was the greatest. The enzyme is inhibited by its substrate, D-fructose, and activity was greatest with a substrate concentration (in the final assay mixture) of 500 mmol/liter and an NADH concentration of 247 mumol/liter. The specimen volume was 100 mul. The enzyme from liver and serum was shown to have similar Km values and activation energies, and we conclude that the liver enzyme was unchanged on passing into the extrahepatic space. Normal values for this assay are 0--2.6 U/liter (2.5 and 97.5 percentiles).
Authors:
C I Rose; A R Henderson
Related Documents :
24189065 - Plant acyl-coa:lysophosphatidylcholine acyltransferases (lpcats) have different specifi...
1009925 - Human-serum cholinesterase subunits and number of active sites of the major component.
4509655 - Qualitative differences in the n-acetyl-d-galactosaminyltransferases produced by human ...
17724765 - Inhibition of non-enzymatic glycation by silk extracts from a mexican land race and mod...
5265 - Reconstituted liver microsomal enzyme system that hydroxylates drugs, other foreign com...
820375 - Evidence for a lipid dependence of mitochondrial nicotinamide nucleotide transhydrogenase.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  21     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1975-12-12     Completed Date:  1975-12-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1619-26     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alcohol Oxidoreductases / blood*,  metabolism
Drug Stability
Evaluation Studies as Topic
Fructose / pharmacology
Heparin / pharmacology
Humans
Hydrogen-Ion Concentration
Kinetics
Liver / enzymology
Methods
NAD / pharmacology
Sorbitol
Temperature
Thermodynamics
Chemical
Reg. No./Substance:
30237-26-4/Fructose; 50-70-4/Sorbitol; 53-84-9/NAD; 9005-49-6/Heparin; EC 1.1.-/Alcohol Oxidoreductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fluorometric enzymatic determination of total cholesterol in serum.
Next Document:  Simple, sensitive method for measuring plasmin and plasminogen activity in plasma.