Document Detail


Reaction of human albumin with aspirin in vitro: mass spectrometric identification of acetylated lysines 199, 402, 519, and 545.
MedLine Citation:
PMID:  19836360     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aspirin esterase activity of human plasma is due to butyrylcholinesterase and albumin. Our goal was to identify the amino acid residues involved in the aspirin esterase activity of albumin. Fatty acid-free human albumin and human plasma were treated with aspirin for 5 min-24 h. Acetylated residues were identified by LC/MS/MS and MALDI-TOF/TOF mass spectrometry of tryptic peptides. Treatment with 0.3 mM aspirin resulted in acetylation of Lys-199, Lys-402, Lys-519, and Lys-545. Treatment with 20 mM aspirin resulted in acetylation of 26 lysines. There was no acetylation of Tyr-411, under any conditions. Acetylated lysine was stable for at least 21 days at pH 7.4, 37 degrees C. Albumin acetylated by aspirin had reduced esterase activity with beta-naphthyl acetate as shown on gels stained for esterase activity. It was concluded that the aspirin esterase activity of albumin is a pseudo-esterase activity in which aspirin stably acetylates lysines and releases salicylate.
Authors:
Mariya S Liyasova; Lawrence M Schopfer; Oksana Lockridge
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-10-27
Journal Detail:
Title:  Biochemical pharmacology     Volume:  79     ISSN:  1873-2968     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-02-23     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  784-91     Citation Subset:  IM    
Copyright Information:
2009 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Aspirin / chemistry*,  pharmacology
Carboxylic Ester Hydrolases / chemistry
Chromatography, High Pressure Liquid
Humans
Lysine / chemistry*,  drug effects
Models, Molecular
Peptide Mapping
Serum Albumin / chemistry*,  drug effects
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Tandem Mass Spectrometry
Grant Support
ID/Acronym/Agency:
CA36727/CA/NCI NIH HHS; P30 CA036727-24S5/CA/NCI NIH HHS; U01 NS058056/NS/NINDS NIH HHS; U01 NS058056-03/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Serum Albumin; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/acetylsalicylic acid hydrolase; K3Z4F929H6/Lysine; R16CO5Y76E/Aspirin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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