Document Detail


Reaction of complement factors varies with prion strains in vitro and in vivo.
MedLine Citation:
PMID:  22222213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Roles of complement factors in prion infection of the central nervous system remain unclear. In this study, we assessed the strain-dependent reactivity of complement factors in prion infections of Neuro2a (N2a) cells and mouse brains. N2a cells persistently infected with either Chandler or 22L scrapie strains were cultured in the presence of normal mouse serum (NMS), followed by staining with phosphatidylserine binding protein and early apoptosis marker Annexin V. The proportion of Annexin V positive cells was increased both in Chandler- and 22L-infected cells. Preincubation of NMS with anti-C1q, C3 and/or C9 antibodies reduced Annexin V positive cells in Chandler-infected cells, while only anti-C3 antibodies were effective on 22L-infected cells. The immunohistochemistry showed that deposition of C1q and C3 was different between Chandler- and 22L-infected mouse brains. These results indicate that the reactivity of complement factors differs between prion strains both in vitro and in vivo.
Authors:
Rie Hasebe; Gregory J Raymond; Motohiro Horiuchi; Byron Caughey
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2012-01-03
Journal Detail:
Title:  Virology     Volume:  423     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-16     Completed Date:  2012-02-29     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  205-13     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institute of Health, Hamilton, MT 59840, USA. r-hasebe@vetmed.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Brain / immunology,  pathology
Cell Line
Complement System Proteins / immunology*
Humans
Mice
Mice, Inbred C57BL
PrPSc Proteins / immunology*
Scrapie / immunology*,  pathology
Grant Support
ID/Acronym/Agency:
ZIA AI000580-20/AI/NIAID NIH HHS; ZIA AI000580-20/AI/NIAID NIH HHS; ZIA AI000580-21/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/PrPSc Proteins; 9007-36-7/Complement System Proteins
Comments/Corrections

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