Document Detail

Reaction of cells cultured in vitro to different asbestos dusts of equal surface area but different fibre length.
MedLine Citation:
PMID:  7066178     Owner:  NLM     Status:  MEDLINE    
Peritoneal macrophages from mice were cultured in Leighton tubes and exposed to UICC chrysotile, crocidolite or amosite. Their cytotoxicity (Erythrosin uptake), release of lactate dehydrogenase (LDH) and uptake of 3H amino acids were determined according to weight (100 micrograms/tube), surface area (21.3 cm2 or fibre length. In all reactions tested chrysotile was more active than the amphiboles, but the latter gained activity if applied according to surface area. Uptake of 3H amino acids basis. Long-fibred asbestos dusts proved more cytotoxic than the corresponding short fibres when used on the basis of equal mass. In experiments with ascites tumour cells induced by nemalite the different asbestos dusts showed a very significant reduction of 3H-labelled thymidine uptake, but on weight basis the amphibole uptake was markedly higher.
J L Kaw; F Tilkes; E G Beck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of experimental pathology     Volume:  63     ISSN:  0007-1021     ISO Abbreviation:  Br J Exp Pathol     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-06-21     Completed Date:  1982-06-21     Revised Date:  2012-05-07    
Medline Journal Info:
Nlm Unique ID:  0372543     Medline TA:  Br J Exp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  109-15     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Asbestos / toxicity*
Ascitic Fluid / cytology
Cells, Cultured
DNA / biosynthesis
L-Lactate Dehydrogenase / metabolism
Macrophages / drug effects*,  metabolism
Reg. No./Substance:
0/Amino Acids; 1332-21-4/Asbestos; 9007-49-2/DNA; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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