| Re-oxygenation improves hypoxia-induced pulp cell arrest. | |
| | |
MedLine Citation:
|
PMID: 16931865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Dental pulp cells can be exposed to hypoxia during severe inflammation or restorative procedures, though their response to hypoxia is not well-understood. We hypothesized that hypoxia has effects on the growth of pulp cells in vitro. When the cells were exposed to hypoxia for 48 hr, cell growth was suppressed, and cell death was detected by Hoechst staining. Western blot analysis revealed that phosphorylation of retinoblastoma protein was inhibited in cells exposed to hypoxia. Analyses of the molecules involved in retinoblastoma protein phosphorylation revealed that hypoxia suppressed cyclin D2 and activated p21(CIP1/WAF1). Further, hypoxia-exposed pulp cells showed improvement of cell viability, cell-cycle progression, and expression of cyclin D2 with re-oxygenation. These findings indicate that hypoxia-induced cell cycle arrest in pulp cells is reversible, while cyclin D2 may play an essential role in the improvement of cell proliferation with re-oxygenation. |
| | |
Authors:
|
Y Ueno; C Kitamura; M Terashita; T Nishihara |
Related Documents
:
|
3397495 - An improved procedure for the isolation of rat brown adipose tissue cells. 17717725 - Photosynthetic oscillation in individual cells of the marine diatom coscinodiscus waile... 15377335 - Counteraction of prb-dependent protection after extreme hypoxia by elevated ribonucleot... 3084075 - Modification of the cytotoxic activity of mitomycin c by oxygen and ascorbic acid in ch... 3605325 - Flow cytometric analysis of intracellular ph in 3t3 cells. 21871175 - Niclosamide induces mitochondria fragmentation and promotes both apoptotic and autophag... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of dental research Volume: 85 ISSN: 0022-0345 ISO Abbreviation: J. Dent. Res. Publication Date: 2006 Sep |
Date Detail:
|
Created Date: 2006-08-25 Completed Date: 2007-01-23 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: United States |
Other Details:
|
Languages: eng Pagination: 824-8 Citation Subset: D; IM |
Affiliation:
|
Division of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Science of Oral Functions, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Animals Apoptosis / drug effects, physiology* Blotting, Western Cell Cycle / drug effects Cell Hypoxia / physiology* Cell Survival / drug effects Cells, Cultured Cyclin D2 Cyclin-Dependent Kinase Inhibitor p21 / metabolism Cyclins / metabolism Dental Pulp / cytology*, metabolism* Oxygen / pharmacology Phosphorylation Rats Retinoblastoma Protein / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Ccnd2 protein, rat; 0/Cdkn1a protein, rat; 0/Cyclin D2; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Retinoblastoma Protein; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of human pulp fibroblasts in angiogenesis.
Next Document: S100A8 triggers oxidation-sensitive repulsion of neutrophils.