| Re-examination of the presence of alpha-lactalbumin in the epididymis of the rat. | |
| | |
MedLine Citation:
|
PMID: 2250249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Using an assay for alpha-lactalbumin in which galactosyltransferase activity was stabilized and a tissue phosphatase inhibitor was present, no evidence was found for alpha-lactalbumin-like activity in rat epididymal tissue, epididymal fluids or medium from cultured epididymal epithelial cells with either glucose or N-acetylglucosamine as acceptor. However, when assay conditions were suboptimal, apparent transfer of radioactivity to both acceptors could be demonstrated in the epididymis and other tissues. In these assays the amount of alpha-lactalbumin registered was linearly correlated to the extent of stimulation of alpha-lactalbumin added exogenously to tissue extracts as internal standards. When rete testis fluid from rats was used as source of galactosyltransferase under suboptimal conditions, no transfer to glucose was demonstrable in epididymal fluid and an apparent decreased transfer to N-acetylglucosamine could be explained by increases in (pyro)phosphatase activity. Putative alpha-lactalbumin activity in the epididymis may be an artefact of unoptimized assays. |
| | |
Authors:
|
M Hölpert; T G Cooper |
Related Documents
:
|
20399779 - Roles of gpr41 and gpr43 in leptin secretory responses of murine adipocytes to short ch... 17980679 - H2s induced hypometabolism in mice is missing in sedated sheep. 17283229 - Pro-inflammatory delipidizing cytokines reduce adiponectin secretion from human adipocy... 16980299 - The alpha-phosphoglucomutase of lactococcus lactis is unrelated to the alpha-d-phosphoh... 15033479 - Aicar stimulates adiponectin and inhibits cytokines in adipose tissue. 6291329 - Exchange of isethionate between blood and tissues in adult and 7-day-old mice. 15749879 - Transplantation tolerance in nf-kappab-impaired mice is not due to regulation but is pr... 1902259 - Isolation, physicochemical characterization and preclinical efficacy evaluation of solu... 11173539 - Alterations of the transforming growth factor-beta signaling pathway in hepatocellular ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of reproduction and fertility Volume: 90 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1990 Nov |
Date Detail:
|
Created Date: 1991-01-17 Completed Date: 1991-01-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 503-14 Citation Subset: IM |
Affiliation:
|
Max Planck Clinical Research Unit for Reproductive Medicine, Münster, Federal Republic of Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylglucosamine
/
metabolism Animals Biological Assay / methods Epididymis / chemistry* Female Glucose / metabolism Lactalbumin / analysis*, blood, metabolism Lactation Male Mammary Glands, Animal / chemistry Pregnancy Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
50-99-7/Glucose; 7512-17-6/Acetylglucosamine; 9013-90-5/Lactalbumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Extension of oestrous cycles and prolonged secretion of progesterone in non-pregnant cattle infused ...
Next Document: Pattern of follicular growth and resumption of ovarian activity in post-partum beef suckler cows.