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Re-evaluation of myoclonin1 immunosignals in neuron, mitotic spindle, and midbody - Nonspecific?
MedLine Citation:
PMID:  23756482     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Mutations in EFHC1 gene cause juvenile myoclonic epilepsy (JME). We previously showed that myoclonin1 protein encoded by EFHC1 is expressed in prenatal choroid plexus and postnatal ependymal cell cilia but may not be in neurons. However, another group reported that myoclonin1 is expressed in neurons and at mitotic spindle, and that the suppression of EFHC1 by RNAi caused disruption of mitotic spindle structure, impaired M-phase progression, and an increase of apoptosis. We re-investigated their results by using the same polyclonal antibody that they used, and found that the signals in neurons remained in Efhc1-deficient mouse, suggesting that the signals in neurons were nonspecific. Furthermore, Efhc1 (-/-) mouse did not show any abnormalities such as disruption of mitotic spindle structure, impaired M-phase progression, and an increase of apoptosis. Further investigations are required to clarify these discrepancies. This article is part of a supplemental special issue entitled Juvenile Myoclonic Epilepsy: What is it Really?
Authors:
Kazuhiro Yamakawa; Toshimitsu Suzuki
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Epilepsy & behavior : E&B     Volume:  28 Suppl 1     ISSN:  1525-5069     ISO Abbreviation:  Epilepsy Behav     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892858     Medline TA:  Epilepsy Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S61-2     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Laboratory for Neurogenetics, RIKEN Brain Science Institute (BSI), 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan. Electronic address: yamakawa@brain.riken.jp.
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