Document Detail


Re-activation of atrophic motor Schwann cells after hypoglossal-facial nerve anastomosis.
MedLine Citation:
PMID:  18337003     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Facial nerve lesions are common in humans and often require surgical intervention. If repair is delayed, reinnervation can be facilitated by transposing the freshly cut hypoglossal nerve end-to-end directly to the distal facial nerve, allowing for uncompromised hypoglossal axons to reinnervate the denervated facial musculature (hypoglossal-facial anastomosis, HFA). Schwann cells (SCs) in the distal nerve stump have an important function in promoting axonal regeneration by expressing multiple regeneration-associated proteins. Chronically denervated SCs cease to express those factors, but it is unknown whether they can be reactivated by fresh axonal sprouts and regain part of their function. We evaluated SC function and viability in distal facial nerve stump of rats at various time points after chronic denervation as well as following immediate or delayed HFA by assessing their expression of growth-associated protein 43 kDa (GAP-43) and the neuregulin receptors erbB2 and erbB4. Our results show that maximal upregulation of those factors in denervated SCs occurred a few weeks after nerve transection, indicating that a short period of denervation might even be beneficial before nerve repair. Motor SCs denervated for 32 weeks had downregulated their activity and ceased to express the regeneration-associated factors. SCs immediately re-expressed GAP-43, erbB2, and erbB4 following contact with fresh hypoglossal motor axons, demonstrating they are competent to promote regeneration even after long-term denervation.
Authors:
Maria Adele Rueger; Sandra Aras; Orlando Guntinas-Lichius; Wolfram F Neiss
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-06
Journal Detail:
Title:  Neuroscience letters     Volume:  434     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-24     Completed Date:  2008-07-10     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  253-9     Citation Subset:  IM    
Affiliation:
Institut I für Anatomie, der Universität zu Köln, D-50924 Köln, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atrophy / physiopathology,  prevention & control,  surgery
Biological Markers / metabolism
Denervation
Facial Nerve / cytology,  physiology*,  surgery
Facial Nerve Injuries / physiopathology,  surgery*
Female
GAP-43 Protein / metabolism
Glycoproteins / metabolism
Growth Cones / physiology,  ultrastructure
Hypoglossal Nerve / cytology,  physiology,  surgery
Immunohistochemistry
Motor Neurons / cytology,  physiology*
Nerve Regeneration / physiology*
Nerve Transfer / methods*
Neuregulin-1 / metabolism
Rats
Rats, Wistar
Receptor, Epidermal Growth Factor / metabolism
Receptor, erbB-2
Recovery of Function / physiology
Schwann Cells / cytology,  physiology*
Time Factors
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Erbb2 protein, rat; 0/GAP-43 Protein; 0/Glycoproteins; 0/Neuregulin-1; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 2.7.10.1/Receptor, erbB-2; EC 2.7.10.1/receptor tyrosine-protein kinase erbB-4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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