Document Detail


RCOR2 is a subunit of the LSD1 complex that regulates ESC property and substitutes for SOX2 in reprogramming somatic cells to pluripotency.
MedLine Citation:
PMID:  21433225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histone demethylase LSD1 can form complex with different Rcor family corepressors in different cell types. It remains unknown if cell-specific Rcor proteins function specifically in distinct cell types. Here, we report that Rcor2 is predominantly expressed in ESCs and forms a complex with LSD1 and facilitates its nucleosomal demethylation activity. Knockdown of Rcor2 in ESCs inhibited ESC proliferation and severely impaired the pluripotency. Moreover, knockdown of Rcor2 greatly impaired the formation of induced pluripotent stem (iPS) cells. In contrast, ectopic expression of Rcor2 in somatic cells together with Oct4, Sox2, and Klf4 promoted the formation of iPS cells. Most interestingly, ectopic expression of Rcor2 in both mouse and human somatic cells effectively substituted the requirement for exogenous Sox2 expression in somatic cell reprogramming.
Authors:
Peng Yang; Yixuan Wang; Jiayu Chen; Hong Li; Lan Kang; Yu Zhang; She Chen; Bing Zhu; Shaorong Gao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  29     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-20     Completed Date:  2011-09-23     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  791-801     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 AlphaMed Press.
Affiliation:
College of Life Science, Peking University, Beijing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / genetics,  metabolism
Animals
Cell Cycle / genetics,  physiology
Cell Line
Cell Proliferation
Hela Cells
Histone Demethylases / genetics,  metabolism*
Humans
Immunohistochemistry
Induced Pluripotent Stem Cells / cytology,  metabolism
Mass Spectrometry
Mice
Microarray Analysis
Nerve Tissue Proteins / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
SOXB1 Transcription Factors / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/SOXB1 Transcription Factors; EC 1.14.11.-/Histone Demethylases; EC 1.5.-/KDM1A protein, human; EC 3.1.3.1/Alkaline Phosphatase

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