Document Detail

Raw and processed fish show identical Listeria monocytogenes genotypes with pulsed-field gel electrophoresis.
MedLine Citation:
PMID:  15954713     Owner:  NLM     Status:  MEDLINE    
A total of 257 raw fish samples at two different sites were examined for the presence of Listeria monocytogenes. The prevalence of L. monocytogenes was 4%. From 11 positive samples, nine different L. monocytogenes pulsed-field gel electrophoresis genotypes were recovered. From nine pulsotypes recovered from raw fish and 32 pulsotypes shown by 101 fish product isolates, two raw fish and fish product pulsotypes were indistinguishable from each other. Although the prevalence of L. monocytogenes in raw fish is low, the range of L. monocytogenes strains entering the processing plant in large amounts of raw material is wide. This indicates that the raw material is an important initial contamination source of L. monocytogenes in fish processing plants. This postulation is supported by the identical pulsotypes recovered from both raw and processed fish. Some L. monocytogenes strains entering a plant may thus contaminate and persist in the processing environment, causing recurrent contamination of the final products via processing machines.
Annukka Markkula; Tiina Autio; Janne Lundén; Hannu Korkeala
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  68     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-15     Completed Date:  2005-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1228-31     Citation Subset:  IM    
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 66, FI-00014, University of Helsinki, Helsinki, Finland.
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MeSH Terms
Electrophoresis, Gel, Pulsed-Field / methods
Equipment Contamination*
Fishes / microbiology
Food Contamination / analysis*,  prevention & control
Food Microbiology
Food-Processing Industry / methods,  standards*
Listeria monocytogenes / genetics,  isolation & purification*
Seafood / microbiology*

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