| Rattus Model Utilizing Selective Pulmonary Ischemia Induces Bronchiolitis Obliterans Organizing Pneumonia. | |
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MedLine Citation:
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PMID: 23364425 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Bronchiolitis obliterans organizing pneumonia (BOOP), a morbid condition when associated with lung transplant and chronic lung disease, is believed to be a complication of ischemia. Our goal was to develop a simple and reliable model of lung ischemia in the Sprague-Dawley rat that would produce BOOP. METHODS: Unilateral ischemia without airway occlusion was produced by an occlusive slipknot placed around the left main pulmonary artery. Studies were performed 7 days later. Relative pulmonary and systemic flow to each lung was measured by injection of Tc-macroaggregated albumin. Histological sections were examined for structure and necrosis and scored for BOOP. Apoptosis was detected by immunohistochemistry with an antibody against cleaved caspase-3. RESULTS: Pulmonary artery blood flow to left lungs was <0.1% of the cardiac output, and bronchial artery circulation was ~2% of aortic artery flow. Histological sections from ischemic left lungs consistently showed Masson bodies, inflammation and young fibroblasts filling the distal airways and alveoli, consistent with BOOP. Quantitative evaluation of BOOP using epithelial changes, inflammation and fibrosis were higher in ischemic left lungs than right or sham-operated left lungs. Apoptosis was increased in areas exhibiting histological BOOP, but there was no histological evidence of necrosis. TLR4 expression was increased in ischemic left lungs over right. CONCLUSIONS: An occlusive slipknot around the main left PA in rats produces BOOP, providing direct evidence that ischemia without immunomodulation or coinfection is sufficient to initiate this injury. It also affords an excellent model to study signaling and genetic mechanisms underlying BOOP. |
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Authors:
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John C Densmore; Paul M Jeziorczak; Anne V Clough; Kirkwood A Pritchard; Breana Cummens; Meetha Medhora; Arjun Rao; Elizabeth R Jacobs |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-28 |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: - ISSN: 1540-0514 ISO Abbreviation: Shock Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Children's Research Institute, Milwaukee, WI 2Clinical and Translational Science Institute, Milwaukee, WI 3Medical College of Wisconsin, Milwaukee, WI 4Marquette University, Milwaukee, WI 5Clement J. Zablocki Veterans Affairs Medical Center, Milwaukee, WI. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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