| Rational strategy for the production of new crude lipases from Candida rugosa. | |
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MedLine Citation:
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PMID: 15928857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis. |
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Authors:
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Pablo Domínguez de María; José M Sánchez-Montero; Andrés R Alcántara; Francisco Valero; José V Sinisterra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology letters Volume: 27 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-06-01 Completed Date: 2005-09-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 499-503 Citation Subset: IM |
Affiliation:
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Grupo de Biotransformaciones, Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad Complutense Madrid, Plaza de Ramón y Cajal, s/n. Ciudad Universitaria, 28040 Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Candida
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enzymology* Dodecanol / metabolism Enzyme Induction Fermentation Glycerol / metabolism Isoenzymes / chemistry, isolation & purification, metabolism Lipase / chemistry, isolation & purification*, metabolism* Oleic Acid / metabolism Oleic Acids / metabolism Plant Oils / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 0/Oleic Acids; 0/Plant Oils; 112-53-8/Dodecanol; 112-80-1/Oleic Acid; 42254-63-7/heptyl oleate; 56-81-5/Glycerol; 8001-21-6/sunflower seed oil; 8001-25-0/olive oil; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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