Document Detail

Rational strategy for the production of new crude lipases from Candida rugosa.
MedLine Citation:
PMID:  15928857     Owner:  NLM     Status:  MEDLINE    
Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis.
Pablo Domínguez de María; José M Sánchez-Montero; Andrés R Alcántara; Francisco Valero; José V Sinisterra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology letters     Volume:  27     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-06-01     Completed Date:  2005-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  499-503     Citation Subset:  IM    
Grupo de Biotransformaciones, Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad Complutense Madrid, Plaza de Ramón y Cajal, s/n. Ciudad Universitaria, 28040 Madrid, Spain.
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MeSH Terms
Candida / enzymology*
Dodecanol / metabolism
Enzyme Induction
Glycerol / metabolism
Isoenzymes / chemistry,  isolation & purification,  metabolism
Lipase / chemistry,  isolation & purification*,  metabolism*
Oleic Acid / metabolism
Oleic Acids / metabolism
Plant Oils / metabolism
Reg. No./Substance:
0/Isoenzymes; 0/Oleic Acids; 0/Plant Oils; 112-53-8/Dodecanol; 112-80-1/Oleic Acid; 42254-63-7/heptyl oleate; 56-81-5/Glycerol; 8001-21-6/sunflower seed oil; 8001-25-0/olive oil; EC

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