| Rational improvement of cell-free protein synthesis. | |
| | |
MedLine Citation:
|
PMID: 20603235 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was used. The performance of different extracts (S12 and S30), the concentration dependence of key components and the effect of different additives were investigated. We find that the initial expression yield can be enhanced twofold to threefold in this manner. The improved conditions comprise a modified S12 extract, optimized concentrations of creatine phosphate and key amino acids, as well as introduction of ketoacid additives. Our results show that current cell-free expression technology is far from optimal and that higher yields and increased utilization of the provided precursors are attainable with further optimization. |
| | |
Authors:
|
Anders Pedersen; Kristofer Hellberg; Johan Enberg; B Göran Karlsson |
Related Documents
:
|
20015835 - Improved alliin yield in somatic embryos of allium sativum l. (cv. yamuna safed) as ana... 1177025 - The fate of four 75se-labeled amino acids: studies of some unsuccessful pancreas-scanni... 11233085 - Response of sugar interconversion, starch and protein accumulation to supplied metaboli... 14074855 - Selenoamino acids: decrease of radiation damage to amino acids and proteins. 14762455 - Iopanoic acid in the management of neonatal graves' disease. 7962785 - The degree of dietary fatty acid unsaturation affects torpor patterns and lipid composi... |
Publication Detail:
|
Type: Journal Article Date: 2010-07-13 |
Journal Detail:
|
Title: New biotechnology Volume: 28 ISSN: 1876-4347 ISO Abbreviation: N Biotechnol Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-04-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101465345 Medline TA: N Biotechnol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 218-24 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Swedish NMR Centre, University of Gothenburg, PO Box 465, SE-40530 Gothenburg, Sweden; Department of Chemistry, University of Gothenburg, PO Box 462, SE-40530 Gothenburg, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A G to C transversion at the last nucleotide of exon 25 of the MYH9 gene results in a missense mutat...
Next Document: The regulation of agricultural biotechnology: science shows a better way.