| Rational development of caged-biotin protein-labeling agents and some applications in live cells. | |
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MedLine Citation:
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PMID: 22035795 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Biotin-(strept)avidin complex is widely used in biotechnology because of its extremely high binding constant, but there is no report describing spatiotemporally controlled formation of the complex in live cells. Here, based on X-ray crystal structure analysis and calorimetric data, we designed and synthesized photoreleasable biotins, which show greatly reduced affinity for (strept)avidin, but recover native affinity after UV irradiation. For application at the cell surface, we introduced an amine-reactive moiety into these "caged" biotin molecules. Specific fluorescence imaging of live cells that had been labeled with these agents and then UV-irradiated, was accomplished by addition of streptavidin conjugated with a fluorophore. We also demonstrated the applicability of these compounds for UV-irradiated-cell-specific drug delivery by using caged-biotin-labeled cells, a prodrug, and streptavidin conjugated with a prodrug-activating enzyme. |
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Authors:
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Takuya Terai; Eri Maki; Shigeru Sugiyama; Yoshinori Takahashi; Hiroyoshi Matsumura; Yusuke Mori; Tetsuo Nagano |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemistry & biology Volume: 18 ISSN: 1879-1301 ISO Abbreviation: Chem. Biol. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9500160 Medline TA: Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1261-72 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; CREST, JST, Sanbancho-blg, 5 Sanbancho, Chiyoda-ku, Tokyo, 102-0075, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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