Document Detail


Rational design, synthesis, and pharmacological evaluation of 2-azanorbornane-3-exo,5-endo-dicarboxylic acid: a novel conformationally restricted glutamic acid analogue.
MedLine Citation:
PMID:  12585893     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The design and synthesis of conformationally restricted analogues of alpha-amino acids is an often used strategy in medicinal chemistry research. Here we present the rational design, synthesis, and pharmacological evaluation of 2-azanorbornane-3-exo,5-endo-dicarboxylic acid (1), a novel conformationally restricted (S)-glutamic acid (Glu) analogue intended as a mimic of the folded Glu conformation. The synthesis of 1 was completed in its racemic form in eight steps from commercially available starting materials. As a key step, the first facially selective hydroboration of a 5-methylidene[2.2.1]bicyclic intermediate was investigated. In this transformation, the catalytic methodology of Wilkinson's/catechol borane proved superior to stoichiometric borane or dialkyl borane reagents, in terms of higher diastereomeric excess and chemical yield. To our surprise (+/-)-1 did not show affinity in binding studies on native 2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid (AMPA) (IC(50) > 300 microM, [(3)H]AMPA) or kainic acid (IC(50) > 160 microM, [(3)H]kainic acid) receptors nor in binding studies on the cloned iGluR5,6 subtypes (IC(50) > 300 microM, [(3)H]kainic acid).
Authors:
Lennart Bunch; Tommy Liljefors; Jeremy R Greenwood; Karla Frydenvang; Hans Bräuner-Osborne; Povl Krogsgaard-Larsen; Ulf Madsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of organic chemistry     Volume:  68     ISSN:  0022-3263     ISO Abbreviation:  J. Org. Chem.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-14     Completed Date:  2003-10-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985193R     Medline TA:  J Org Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1489-95     Citation Subset:  IM    
Affiliation:
Department of Medicinal Chemistry, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark. lebu@dfh.dk
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MeSH Terms
Descriptor/Qualifier:
Aza Compounds / chemical synthesis*,  pharmacology*
Dicarboxylic Acids / chemical synthesis*,  pharmacology*
Glutamic Acid* / analogs & derivatives,  chemistry,  pharmacology
Inhibitory Concentration 50
Kainic Acid / pharmacology
Magnetic Resonance Spectroscopy
Molecular Conformation
Molecular Mimicry
Molecular Structure
Norbornanes / chemical synthesis*,  pharmacology*
Receptors, Glutamate / chemistry*,  metabolism*
Stereoisomerism
Structure-Activity Relationship
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology
Chemical
Reg. No./Substance:
0/2-azanorbornane-3-exo,5-endo-dicarboxylic acid; 0/Aza Compounds; 0/Dicarboxylic Acids; 0/Norbornanes; 0/Receptors, Glutamate; 487-79-6/Kainic Acid; 56-86-0/Glutamic Acid; 77521-29-0/alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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