Document Detail

Rational design of a protein-based molecular device consisting of blue fluorescent protein and zinc protoporphyrin IX incorporated into a cytochrome b562 scaffold.
MedLine Citation:
PMID:  15000478     Owner:  NLM     Status:  MEDLINE    
To make a single molecular photo-device, it is essential to control the exact orientation of two types of proteins. We made a chimeric protein in which cytochrome b562 was linked to the N-terminus of enhanced green fluorescent protein, cytb562-EGFP. Within cytb562-EGFP, the excitation energy of EGFP was transferred to the cytochrome b562 cofactor fixed proximally to EGFP. Cytb562-EGFP was engineered so that iron protoporphyrin IX was substituted by zinc protoporphyrin IX to make it a suitable cofactor for photo-induced electron transfer. The photosensitizer pigment was optimized and the EGFP was replaced by a blue fluorescent mutant that gave 15% higher energy transfer efficiency.
Shuji Takeda; Noriho Kamiya; Teruyuki Nagamune
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology letters     Volume:  26     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-03-05     Completed Date:  2004-09-21     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  121-5     Citation Subset:  IM    
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-8656, Japan.
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MeSH Terms
Cytochrome b Group / chemistry*
Energy Transfer
Escherichia coli Proteins / chemistry*
Green Fluorescent Proteins
Luminescent Proteins / chemistry*
Models, Molecular
Protein Conformation
Protein Engineering
Protoporphyrins / chemistry*
Recombinant Fusion Proteins / chemistry
Spectrometry, Fluorescence
Reg. No./Substance:
0/Cytochrome b Group; 0/Escherichia coli Proteins; 0/Luminescent Proteins; 0/Protoporphyrins; 0/Recombinant Fusion Proteins; 0/blue fluorescent protein, Aequorea victoria; 0/iron protoporphyrin IX; 147336-22-9/Green Fluorescent Proteins; 15442-64-5/zinc protoporphyrin; 9064-79-3/cytochrome b562, E coli

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