Document Detail


Rates of utilization and fates of glucose, glutamine, pyruvate, fatty acids and ketone bodies by mouse macrophages.
MedLine Citation:
PMID:  3593269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The concentrations of ATP and the ATP/AMP concentration ratios were maintained in thioglycollate-elicited mouse peritoneal macrophages incubated in vitro for 90 min in the presence or absence of added substrate: rates of glycolysis, lactate formation and glutamine utilization were approximately linear with time for at least 60 min of incubation. The rate of oxygen consumption by macrophages was only increased above the basal rate (i.e. that in the absence of added substrate) by addition of succinate or pyruvate, or by addition of the uncoupling agent carboxyl cyanide m-chlorophenylhydrazone ('CCCP'); it was decreased by 75% by the addition of KCN. These findings suggest that metabolism of endogenous substrate can provide most, if not all, of the energy requirement of these cells, at least for a short period. The rates of glucose and glutamine utilization by incubated macrophages were approx. 300 and 100 nmol/min per mg of protein respectively. A large proportion of the glutamine that is utilized is converted into glutamate and aspartate, and very little (perhaps less than 10%) is oxidized. Similarly almost all of the glucose that is utilized is converted into lactate and very little is oxidized. This characteristic is similar to that of resting lymphocytes and rapidly dividing cells; in non-proliferating macrophages it may be a mechanism to provide precision in control of the rate of biosynthetic processes that utilize intermediates of these pathways, e.g. purines and pyrimidines for mRNA for the synthesis of secretory proteins and glycerol 3-phosphate for phospholipid synthesis for membrane recycling. No utilization of acetoacetate or 3-hydroxybutyrate by macrophages was detected. In contrast, both butyrate and oleate were oxidized. The rate of [14C]oleate conversion into 14CO2 (1.3 nmol/h per mg of protein) could account for most of the oxygen consumption by incubated macrophages, suggesting that long-chain fatty acids might provide an important fuel in situ. This may be one explanation for the secretion of lipoprotein lipase by these cells, to provide fatty acids for oxidation from the degradation of local triacylglycerol.
Authors:
P Newsholme; S Gordon; E A Newsholme
Related Documents :
15611129 - Peroxisomal and mitochondrial oxidation of fatty acids in the heart, assessed from the ...
19950509 - Oxidation of oleic acid at the air-water interface and its potential effects on cloud c...
23297069 - Improvement of free fatty acid production in escherichia coli using codon-optimized str...
7986209 - Aminotriazole is a potent inhibitor of alpha-oxidation of 3-methyl-substituted fatty ac...
2835769 - Isolation and characterization of natural allene oxides: unstable intermediates in the ...
8283379 - A new syndrome with ethylmalonic aciduria and normal fatty acid oxidation in fibroblasts.
17375469 - Cytochemical localization of acid phosphatase in stigeoclonium tenue (chaetophorales, c...
7708809 - Inhibition of 15-hydroxy prostaglandin dehydrogenase activity in rabbit gastric antral ...
19502729 - Glutamyl trna synthetases and glutamic acid induce sexual differentiation of schizosacc...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  242     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-07-22     Completed Date:  1987-07-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  631-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides / metabolism
Animals
Aspartic Acid / metabolism
Fatty Acids / metabolism*
Female
Glucose / metabolism*
Glutamine / metabolism*
Ketone Bodies / metabolism*
Lactates / metabolism
Lactic Acid
Macrophages / metabolism*
Mice
Mice, Inbred C57BL
Oxygen Consumption
Pyruvates / metabolism*
Pyruvic Acid
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 0/Fatty Acids; 0/Ketone Bodies; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 50-99-7/Glucose; 56-84-8/Aspartic Acid; 56-85-9/Glutamine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adenine nucleotide metabolism in isolated chicken hepatocytes.
Next Document:  Properties and distribution of the protein inhibitor (Mr 17,000) of protein kinase C.