Document Detail

Rates of generation of methotrexate-resistant variants in cells temperature-sensitive for malignant transformation.
MedLine Citation:
PMID:  3368829     Owner:  NLM     Status:  MEDLINE    
We used tsLA23-NRK cells, in which malignant transformation is under the control of a temperature-sensitive (ts) src oncogene, to examine the relationship between genetic instability and malignant transformation. Cells were maintained in vitro as either transformed cells (at 36 degrees C) or as normal cells (at 39 degrees C). Genetic instability was assessed in these two cell populations by determining (1) the frequencies of methotrexate (MTX)-resistant variants present in these populations, and (2) the rates of generation of MTX-resistant variants, as determined from Luria-Delbrück fluctuation analyses. We observed no significant differences, in either of these parameters of genetic instability, that could be attributed to transformation status. We conclude that in this defined cellular system there is no obligatory relationship between malignant transformation and increased genetic instability, as assessed by either frequencies or rates of generation of variants resistant to MTX.
A F Chambers; J F Harris; J S Grundy
Related Documents :
6307919 - Herpes simplex virus (hsv) antigens on hsv-transformed rat cells can be demonstrated by...
3028939 - Frequency of b-lymphocyte transformation by epstein-barr virus decreases with entry int...
2987679 - Gene transfer method for transient gene expression, stable transformation, and cotransf...
25206489 - Role of endogenous schwann cells in tissue repair after spinal cord injury.
6258989 - Behaviour of normal and neoplastic rat cells on grooved substrates.
25352009 - Vaccarin attenuates the human ea.hy926 endothelial cell oxidative stress injury through...
22361729 - Activation of two distinct sox9-egfp-expressing intestinal stem cell populations during...
18417179 - 1-nitropyrene (1-np) induces apoptosis and apparently a non-apoptotic programmed cell d...
7554099 - Bivascular liver perfusion in the anterograde and retrograde modes: zonation of the res...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Somatic cell and molecular genetics     Volume:  14     ISSN:  0740-7750     ISO Abbreviation:  Somat. Cell Mol. Genet.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-06-14     Completed Date:  1988-06-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8403568     Medline TA:  Somat Cell Mol Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  253-9     Citation Subset:  IM    
London Regional Cancer Centre, Ontario, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Transformation, Neoplastic* / drug effects
Drug Resistance
Methotrexate / pharmacology*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Aspergillosis in a healthy host.
Next Document:  Determination of the right ventricular volume and ejection fraction by echocardiography (a compariso...