Document Detail

Rates of cycling cells in cryopreserved valvular homograft: a preliminary study.
MedLine Citation:
PMID:  17298405     Owner:  NLM     Status:  MEDLINE    
Some investigators claim that the viability of cryopreserved human valvular homograft is necessary for the duration of implanted homograft. In this preliminary study, the percentage of cycling cells in cryopreserved valvular homografts was evaluated with the use of monoclonal Ki-67 antibody. Three human aortic valves were harvested from multiorgan donors and cryopreserved. Sections of 5 microm in thickness were stained with monoclonal Ki-67 antibody. The proportion of endothelial cells with Ki-67 positive nuclei was 1.80 +/- 0.20%. No differences in distribution were observed from basal to marginal sites. Few fibroblasts showed Ki-67-immunopositivity (0.10 +/- 0.06%) while the Ki-67 immunostaining was 0.80 +/- 0.20% in myocytes. Our preliminary study shows that cryopreserved valvular homograft cells are not only viable but they also have the potential to replicate. These data can lead to the hypothesis that valvular cells could actively replicate even after implantation, permitting cellular renewal and regeneration of extracellular matrix's components.
Fabio Barili; Luca Dainese; Faisal H Cheema; Giacomo Dell'Antonio; Veli K Topkara; Giuseppe Rossoni; Anna Guarino; Barbara Micheli; Claudio Doglioni; Paolo Biglioli; Gianluca Polvani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Artificial organs     Volume:  31     ISSN:  0160-564X     ISO Abbreviation:  Artif Organs     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-14     Completed Date:  2007-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  152-4     Citation Subset:  IM    
Approved Regional Tissue Bank, Department of Cardiovascular Surgery, University of Milan, Centro Cardiologico Monzino IRCCS, Milan, Italy.
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MeSH Terms
Cell Survival
Endothelial Cells / metabolism
Heart Valves / transplantation*
Ki-67 Antigen / metabolism
Middle Aged
Transplantation, Homologous
Reg. No./Substance:
0/Ki-67 Antigen

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