Document Detail

Rates of aggregation, loss of anchorage dependence, and tumorigenicity of cultured cells.
MedLine Citation:
PMID:  189308     Owner:  NLM     Status:  MEDLINE    
The net rate of spontaneous aggregation of cells suspended with EDTA was measured for various cell types including spontaneous transformants and cells transformed with DNA and RNA viruses. The anchorage dependence as determined by growth in methyl cellulose and the tumorigenicity in vivo were also determined. All cells that had lost their anchorage dependency and were tumorigenic showed a high net rate of spontaneous adhesion. A31 was the only nontransformed cell line to have a high net rate of adhesion. The net rate of spontaneous aggregation of cells is a quick and reliable index of tumorigenicity and offers a new approach to understanding the mechanisms of cell surface changes associated with transformation.
T C Wright; T E Ukena; R Campbell; M J Karnovsky
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  74     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-03-21     Completed Date:  1977-03-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  258-62     Citation Subset:  IM    
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MeSH Terms
Cell Adhesion*
Cell Aggregation* / drug effects
Cell Line
Cell Transformation, Neoplastic*
Cells, Cultured
Concanavalin A / pharmacology
Edetic Acid / pharmacology
Neoplasms, Experimental / pathology,  physiopathology*
Sarcoma Viruses, Murine
Simian virus 40
Reg. No./Substance:
11028-71-0/Concanavalin A; 60-00-4/Edetic Acid; 9004-67-5/Methylcellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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