Document Detail

Rate of metabolic decarboxylation of leucine as assessed by a l[1-(13)C(1)]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio.
MedLine Citation:
PMID:  17615007     Owner:  NLM     Status:  MEDLINE    
Research has shown that broiler chickens reared on a low-protein diet have a more efficient protein digestion. However, information on the fate of absorbed amino acids in relation to the dietary crude protein level in poultry is sparse. Therefore, this study aimed at developing a methodology for a 1-(13)C(1)-leucine breath test combined with indirect calorimetry, and to apply this technique using broiler diets known to induce differences in protein retention. From 14 days of age onwards, broiler chickens were reared on one of two isocaloric diets with substitutions between fat and protein [low-protein (LP) vs. high-protein (HP) diet: 130.4 vs. 269 g protein/kg; and 101.8 vs. 27.9 g fat/kg]. Every 4 or 5 days, three chickens per diet were placed in the respiratory cells for 48 h. The broilers were intubated with 40 mg 1-(13)C(1)-leucine/kg body weight, followed by breath sampling for 4 h at 15-min intervals and mass spectrometric analysis of the (13)C:(12)C ratio in the samples. The CO(2) level in the respiratory cell air was monitored and excreta samples were collected. The methodology to study l[1-(13)C(1)]leucine decarboxyation in chickens using a breath test combined with indirect calorimetry was accomplished. Results of the nitrogen balance test indicated that the LP broilers had an improved dietary protein retention compared with the HP animals. Moreover, LP chickens decarboxylated a significantly lower percentage of l[1-(13)C(1)]leucine, demonstrating several 'protein- or amino acid-sparing' mechanisms in animals reared on a diet with lower protein level, both at the digestive and at the postabsorptive level.
Q Swennen; C Laroye; G Janssens; K Verbeke; E Decuypere; J Buyse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal physiology and animal nutrition     Volume:  91     ISSN:  0931-2439     ISO Abbreviation:  J Anim Physiol Anim Nutr (Berl)     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-06     Completed Date:  2007-09-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101126979     Medline TA:  J Anim Physiol Anim Nutr (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  347-54     Citation Subset:  IM    
Laboratory of Livestock Physiology, Immunology and Genetics, Department of Biosystems, K.U.Leuven, Heverlee, Belgium.
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MeSH Terms
Aging / metabolism,  physiology
Animal Feed
Area Under Curve
Body Weight / physiology
Breath Tests*
Calorimetry, Indirect / methods,  veterinary*
Carbon Isotopes
Chickens / metabolism*
Dietary Fats / administration & dosage,  metabolism
Dietary Proteins / administration & dosage*,  metabolism*
Dose-Response Relationship, Drug
Leucine / administration & dosage,  metabolism
Random Allocation
Reg. No./Substance:
0/Carbon Isotopes; 0/Dietary Fats; 0/Dietary Proteins; 61-90-5/Leucine

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