Document Detail


Rat vena cava alpha 1B-adrenoceptors: characterization by [3H]prazosin binding and contraction experiments.
MedLine Citation:
PMID:  8223950     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined which subtypes of alpha 1-adrenoceptors are expressed in rat vena cava by using both functional and [3H]prazosin binding experiments. Pretreatment with chloroethylclonidine inactivated about 80% of the specific [3H]prazosin binding sites and reduced the maximal noradrenaline-induced contraction to the same extent. Competition with subtype-selective agonists and antagonists showed primarily the alpha 1B-adrenoceptor subtype in vena cava. The number of alpha 1-adrenoceptors estimated with [3H]prazosin binding and the maximal noradrenaline-induced contraction were dose-dependently inhibited by phenoxybenzamine, indicating the absence of receptor reserve for noradrenaline in vena cava. As the noradrenaline-induced contraction was largely inhibited in Ca(2+)-free solution, these results suggest that alpha 1B-adrenoceptors can be mainly linked to Ca2+ influx in rat vena cava.
Authors:
I Sayet; G Neuilly; L Rakotoarisoa; J Mironneau; C Mironneau
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  246     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-12-17     Completed Date:  1993-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  275-81     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Cellulaire et Pharmacologie Moléculaire, URA CNRS 1489, Université de Bordeaux II, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Norepinephrine / pharmacology
Prazosin / metabolism*
Radioligand Assay
Rats
Receptors, Adrenergic, alpha-1 / metabolism*
Tritium / diagnostic use
Vasoconstriction / drug effects*
Venae Cavae / drug effects*,  metabolism,  physiology
Chemical
Reg. No./Substance:
0/Receptors, Adrenergic, alpha-1; 10028-17-8/Tritium; 19216-56-9/Prazosin; 51-41-2/Norepinephrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of HOE 694--a novel inhibitor of Na+/H+ exchange--on NIH 3T3 fibroblasts expressing the RAS ...
Next Document:  Expression and properties of recombinant alpha 1 beta 2 gamma 2 and alpha 5 beta 2 gamma 2 forms of ...