Document Detail


Rat sertoli cells: a rapid method for obtaining viable cells.
MedLine Citation:
PMID:  163729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described for obtaining populations of viable Sertoli cells from rat testes. Minced whole testes from rats of 15 to 29 days of age are sequentially treated with collagenase and pancreatin. The resulting suspension of cells is sedimented through a sucrose density gradient. Preparations are produced consisting of from 60% to 82% Sertoli cells, an enrichment of 2 to 5 times the proportion of Sertoli cells in whole testes of these ages. The preparations are free of interstitial cells, are essentially free of peritubular cells and contain reduced numbers of germinal cells; the main contaminating cell types are spermatogonia and spermatocytes. The Sertoli cells are considered to be 95% viable by their ability to exclude trypan blue and by subsequent culturing in vitro. The entire procedure requires 3 h. Maintenance of the Sertoli-enriched fraction in modified Eagle's minimal essential medium temporarily at 41 C allows preparations of Sertoli cell monolayer cultures consisting of 95%-98% Sertoli cells within 3 days.
Authors:
M J Welsh; J P Wiebe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrinology     Volume:  96     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-06-09     Completed Date:  1975-06-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  618-24     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods*
Cells, Cultured
Centrifugation, Density Gradient
Male
Microbial Collagenase
Pancreatin
Rats
Spermatozoa
Testis / cytology*
Chemical
Reg. No./Substance:
8049-47-6/Pancreatin; EC 3.4.24.3/Microbial Collagenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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