Document Detail

Rat sertoli cells: a rapid method for obtaining viable cells.
MedLine Citation:
PMID:  163729     Owner:  NLM     Status:  MEDLINE    
A method is described for obtaining populations of viable Sertoli cells from rat testes. Minced whole testes from rats of 15 to 29 days of age are sequentially treated with collagenase and pancreatin. The resulting suspension of cells is sedimented through a sucrose density gradient. Preparations are produced consisting of from 60% to 82% Sertoli cells, an enrichment of 2 to 5 times the proportion of Sertoli cells in whole testes of these ages. The preparations are free of interstitial cells, are essentially free of peritubular cells and contain reduced numbers of germinal cells; the main contaminating cell types are spermatogonia and spermatocytes. The Sertoli cells are considered to be 95% viable by their ability to exclude trypan blue and by subsequent culturing in vitro. The entire procedure requires 3 h. Maintenance of the Sertoli-enriched fraction in modified Eagle's minimal essential medium temporarily at 41 C allows preparations of Sertoli cell monolayer cultures consisting of 95%-98% Sertoli cells within 3 days.
M J Welsh; J P Wiebe
Related Documents :
185309 - Purification and characterization of leydig cells from rat testes.
16728019 - Increased germ cell degeneration and reduced germ cell:sertoli cell ratio in stallions ...
1664679 - Mannose 6-phosphate receptors: potential mediators of germ cell-sertoli cell interactions.
19268429 - Deprival of testicular innervation induces apoptosis of leydig cells via caspase-8-depe...
2827999 - Age-related and testicular regression-induced changes in adenosine 3',5'-monophosphate ...
9138629 - Leydig cell hyperplasia and adenoma formation: mechanisms and relevance to humans.
2578889 - Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal c...
23404059 - Peripheral blood mononuclear cells inhibit proliferation and promote apoptosis of hela ...
15107589 - Herg k channel conductance promotes h2o2-induced apoptosis in hek293 cells: cellular me...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrinology     Volume:  96     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-06-09     Completed Date:  1975-06-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  618-24     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Separation / methods*
Cells, Cultured
Centrifugation, Density Gradient
Microbial Collagenase
Testis / cytology*
Reg. No./Substance:
8049-47-6/Pancreatin; EC Collagenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Differences in corticosterone and dexamethasone binding to rat brain and pituitary.
Next Document:  A protein from polymorphonuclear leukocytes (LEM) which affects the rate of hepatic amino acid trans...