Document Detail


Rat renal prorenin concentration in experimental hypertension.
MedLine Citation:
PMID:  11131044     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amount of renal prorenin in models of hypertension in rats was studied by using a novel enzyme (PreR-Co). Ten microgrames of PreR-Co promoted a complete conversion of inactive renin, and during the first 15-min incubation the reaction was under initial velocity conditions. The enzyme-substrate reaction obeyed Michaelis-Menten kinetics, with a Vmax of 0.97 x 10(-5) pmol Ang I/min and a Km of 5.03 x 10(-5) pmol prorenin. The difference between the total renin concentration (TRC) and active renin concentration (ARC) in the normal rat kidney (356.4 +/- 20.6 and 105.3 +/- 7.6 ng Ang I/mg tissue/h respectively), indicated that inactive renin comprised 70% of TRC. In the aortic coarctation model, inactive renin comprised 68 % of TRC in the right kidney and no or very little prorenin was found in the left kidney. In the Goldblatt 2-kidney, 1-clip rats, the right kidney prorenin comprised 61% of the TRC and 54% in the clamped left kidney. After DOCA-Salt treatment prorenin was almost absent in the rat kidneys. In conclusion, we have developed an easy and sensitive method to measure inactive renin in the kidney that may be useful to study the biochemical events of renin maturation in physiological and pathological states.
Authors:
P A Vincent; E De Vito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental hypertension (New York, N.Y. : 1993)     Volume:  22     ISSN:  1064-1963     ISO Abbreviation:  Clin. Exp. Hypertens.     Publication Date:    2000 Oct-Nov
Date Detail:
Created Date:  2000-12-22     Completed Date:  2001-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9305929     Medline TA:  Clin Exp Hypertens     Country:  United States    
Other Details:
Languages:  eng     Pagination:  675-86     Citation Subset:  IM    
Affiliation:
Instituto Superior de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aortic Coarctation / metabolism
Desoxycorticosterone
Enzyme Precursors / metabolism*
Female
Hypertension / chemically induced,  metabolism*
Hypertension, Renovascular / metabolism
Kidney / drug effects,  metabolism*
Kinetics
Male
Osmolar Concentration
Rats
Rats, Sprague-Dawley
Reference Values
Renal Artery Obstruction / metabolism
Renin / metabolism*
Sodium Chloride
Chemical
Reg. No./Substance:
0/Enzyme Precursors; 64-85-7/Desoxycorticosterone; 7647-14-5/Sodium Chloride; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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