Document Detail


Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells.
MedLine Citation:
PMID:  11359947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human marrow stromal cell (hMSCs) were recently shown to expand rapidly in culture when plated at a low density of approximately 3 cells/cm(2). Low-density plating promoted proliferation of small recycling stem (RS) cells that appeared to be the most multipotent cells in the cultures. Here we demonstrated that MSCs from rat bone marrow (rMSCs) are even more sensitive to low-density plating than hMSCS: When plated at approximately 2 cells/cm(2), the cells expanded over 4,000-fold in 12 days, over twice the maximal rate observed with hMSCS: Analysis by fluorescence-activated cell sorter demonstrated that rMSCs had the same heterogeneity seen with hMSCs in that the cultures contained both small rapidly RS cells and much larger mature cells (mMSCs). The rat mMSCs differed from human mMSCs in that they regenerated RS cells in culture. Also, after low-density plating, colonies of rMSCs expanded into confluent cultures, whereas colonies of hMSCs did not.
Authors:
E H Javazon; D C Colter; E J Schwarz; D J Prockop
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  19     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2001  
Date Detail:
Created Date:  2001-05-21     Completed Date:  2001-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-25     Citation Subset:  IM    
Affiliation:
Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells / cytology*
Cell Culture Techniques / methods*
Cell Differentiation
Cell Division
Cell Separation
Cells, Cultured
Epitopes
Flow Cytometry
Green Fluorescent Proteins
Humans
Luminescent Proteins / metabolism
Microscopy, Phase-Contrast
Osteogenesis
Rats
Rats, Inbred Lew
Recombinant Fusion Proteins / metabolism
Stromal Cells / cytology*
Time Factors
Transduction, Genetic
Chemical
Reg. No./Substance:
0/Epitopes; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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