| Rat lens cultures: MIP expression and domains of intercellular coupling. | |
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MedLine Citation:
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PMID: 3700025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple means of establishing a differentiating rat lens culture system is presented which exhibits a high degree of both morphologic and biochemical differentiation along lens-specific lines. Morphological differentiation includes cell enlargement, displacement from the cell substratum, and the loss of intracellular organelles. Biochemical differentiation includes the de novo synthesis of the Main Intrinsic Polypeptide (MIP), a putative fiber cell junctional polypeptide, as well as the synthesis of the beta and gamma crystallins. Mapping of domains of intercellular communication as defined by the spread of intracellularly injected Lucifer Yellow CH and electrical coupling, are compared to the distribution of biochemically and morphologically differentiated cells. |
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Authors:
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P G FitzGerald; D A Goodenough |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 27 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1986 May |
Date Detail:
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Created Date: 1986-06-02 Completed Date: 1986-06-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 755-71 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aquaporins Electrophysiology Eye Proteins / metabolism*, physiology Intercellular Junctions / metabolism, physiology Lens, Crystalline / metabolism*, physiology, ultrastructure Membrane Glycoproteins* Rats |
| Grant Support | |
ID/Acronym/Agency:
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EY 02430/EY/NEI NIH HHS; EY05642/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aquaporins; 0/Eye Proteins; 0/Membrane Glycoproteins; 0/aquaporin 0 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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