Document Detail


Rat hepatic glutathione S-transferase-mediated embryotoxic bioactivation of ethylene dibromide.
MedLine Citation:
PMID:  1462248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The embryotoxic effects of ethylene dibromide (EDB) bioactivation, mediated by purified rat liver glutathione S-transferases (GST), were investigated using rat embryos in culture. Significant EDB metabolism was observed with rat liver GST purified by affinity chromatography (specific activity of 188 +/- 11.3 nmol/min/mg protein). The reaction was enzymatic in nature and the conjugation rate was proportional to the concentration of EDB (up to 0.75 mM) and the enzyme present in the reaction medium. EDB activation by 100 units (1 unit = 1 nmol of glutathione consumed per min) of purified rat liver GST caused a significant reduction in general development as measured by crown-rump length, yolk sac diameter, somite number, and the composite score for different morphological parameters (Brown and Fabro methodology). Structures most significantly affected were the central nervous and olfactory systems as well as the yolk sac circulation and allantois. The results of this study clearly indicate that under in vitro conditions, bioactivation of EDB by GST can lead to embryotoxicity.
Authors:
A Mitra; D R Hilbelink; J J Dwornik; A Kulkarni
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Teratology     Volume:  46     ISSN:  0040-3709     ISO Abbreviation:  Teratology     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-01-12     Completed Date:  1993-01-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0153257     Medline TA:  Teratology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  439-46     Citation Subset:  IM    
Affiliation:
Florida Toxicology Research Center, College of Public Health, University of South Florida, Tampa 33612.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biotransformation
Culture Techniques
Embryo, Mammalian / drug effects*,  metabolism
Embryonic and Fetal Development / drug effects
Ethylene Dibromide / metabolism,  pharmacokinetics,  toxicity*
Female
Glutathione Transferase / metabolism
Liver / metabolism
Pregnancy
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
106-93-4/Ethylene Dibromide; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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