Document Detail

Rat hair follicle-constituting cells labeled by a newly-developed somatic stem cell-recognizing antibody: a possible marker of hair follicle development.
MedLine Citation:
PMID:  23275308     Owner:  NLM     Status:  In-Data-Review    
A3 was generated as an antibody recognizing somatic stem cells in rat tissues. We investigated the distribution of A3-positive cells in developing rat hair follicles by immunolabeling. A3-positive cells began to be seen in the hair germ and peg in fetuses and neonates; the positive cells were epithelial cells above basal cells. Furthermore, A3-positive cells were seen in the outer root sheath adjacent to the bulge in mature hair follicles. Double immunofluorescence revealed that these A3-positive epithelial cells reacted to E-cadherin (for all epithelial elements) but not to CK15 (for basal cells/epithelial stem cells) or to nestin (for stem cells), indicating that A3-positive epithelial cells are suprabasal cells in the developing epidermic hair follicle. Additionally, spindle-shaped mesenchymal cells surrounding the hair peg and mature hair follicle reacted to A3; in double immunofluorescence, the A3-positive cells were located outside collagen type IV-positive glassy membrane, and reacted to vimentin (for mesenchmal cells), Thy-1 (for immature mesenchymal cells), CD34 (for stem cells) and nestin, but not to α-smooth muscle actin (for myofibroblasts); the positive cells were regarded as immature mesenchymal cells with stem cell nature in the connective tissue sheath of developing hair follicles. A3-positive epithelial and mesenchymal cells did not show proliferating activity. Collectively, it is considered that A3-positive cells seen in developing rat hair follicles may be quiescent post-progenitor cells with the potential to differentiate into either highly-differentiated epithelial or mesenchymal cells. A3 would become a useful antibody to know the kinetics of rat hair follicle-constituting cells.
C Ichikawa; T Izawa; V Juniantito; M Tanaka; M Hori; K Tanaka; S Takenaka; M Kuwamura; J Yamate
Related Documents :
24414098 - Degradation of the endosperm cell walls of lactuca sativa l., cv. grand rapids : timing...
23428168 - Adherence inhibition of enteropathogenic escherichia coli by chitooligosaccharides with...
24552808 - Cyld coordinates with eb1 to regulate microtubule dynamics and cell migration.
24465798 - Msln gene silencing has an anti-malignant effect on cell lines overexpressing mesotheli...
17283068 - Involvement of dihydroceramide desaturase in cell cycle progression in human neuroblast...
24533688 - Induction of p21(waf1/cip1) by garcinol via downregulation of p38-mapk signaling in p53...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histology and histopathology     Volume:  28     ISSN:  1699-5848     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  257-68     Citation Subset:  IM    
Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano-shi, Osaka, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The effect of glucagon and cyclic adenosine monophosphate on acute liver damage induced by acetamino...
Next Document:  A comparative study of extracellular matrix remodeling in two murine models of emphysema.