| Rat factor X is synthesized as a single chain precursor inducible by prothrombin fragments. | |
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MedLine Citation:
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PMID: 7130196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Factor X in plasma is a gamma-carboxylated two-chain glycoprotein which, in activated form, plays a pivotal role in blood coagulation. We have utilized purified rat Factor X antibody, coupled to Sepharose, to isolate and characterize Factor X in rat liver, plasma, and hepatoma cells. Rat factor X is synthesized as a single chain precursor (Mr = 63,000). It is this form which undergoes vitamin K-dependent carboxylation in rat liver microsomes. Only after secretion is Factor X converted into its two-chain mature form. Single chain X synthesis and secretion in hepatoma cells is enhanced by vitamin K. The amount of single chain X secreted by these cells is one-half that of prothrombin. The NH2-terminal gamma-carboxylated fragments of prothrombin which induce prothrombin synthesis (Graves, C. B., Munns, T. W., Carlisle, T. L., Grant, G. A., and Strauss, A. W. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 4772-4776) also induce single chain X synthesis by hepatoma cells. We propose that synthesis of all vitamin K-dependent proteins may be regulated by this common control mechanism. |
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Authors:
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C B Graves; T W Munns; A K Willingham; A W Strauss |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 257 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1982-12-18 Completed Date: 1982-12-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 13108-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Electrophoresis, Polyacrylamide Gel Factor X / biosynthesis*, isolation & purification Liver Neoplasms, Experimental / metabolism* Peptide Fragments / metabolism Prothrombin / physiology* Rats Tunicamycin / pharmacology Vitamin K / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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AM 20407/AM/NIADDK NIH HHS; HL 15619/HL/NHLBI NIH HHS; HL 20577/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Peptide Fragments; 11089-65-9/Tunicamycin; 12001-79-5/Vitamin K; 9001-26-7/Prothrombin; 9001-29-0/Factor X |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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