Document Detail


Rat exocrine pancreas following total gastrectomy.
MedLine Citation:
PMID:  2445872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sixteen rats underwent a total gastrectomy with an end-to-end anastomosis between the esophagus and duodenum. After either two or four weeks, the entire pancreas was excised and isolated lobules were incubated for 60 min in Krebs-Ringer-Hepes solution either with or without addition of ceruletide (5 X 10(-10) g/ml). Amylase was determined every 15 min in the supernatant medium. Measurements of amylase, lipase, trypsin, DNA and total protein were carried out in the homogenized pancreatic tissue. Morphometry was performed via light microscopic analysis. Two weeks following gastrectomy there was an increase in pancreatic wet weight, DNA, total protein and enzyme content (except lipase). Four weeks after surgery, pancreatic weight, amylase and trypsin increased further, whereas DNA content and lipase content remained stable. The DNA concentration decreased, and the size of exocrine pancreatic cells increased. In vitro, amylase discharge increased only after four weeks. The experiments demonstrate a short-term adaptation of the rat exocrine pancreas following total gastrectomy in the form of cellular hyperplasia (after 2 weeks), and hypertrophy (after 4 weeks), and enzyme dissociation.
Authors:
M Büchler; P Malfertheiner; B Glasbrenner; H G Beger
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  International journal of pancreatology : official journal of the International Association of Pancreatology     Volume:  1     ISSN:  0169-4197     ISO Abbreviation:  Int. J. Pancreatol.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1988-01-06     Completed Date:  1988-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8703511     Medline TA:  Int J Pancreatol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  389-98     Citation Subset:  IM    
Affiliation:
Department of Surgery, University of Ulm, F.R.G.
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MeSH Terms
Descriptor/Qualifier:
Amylases / metabolism
Animals
DNA / metabolism
Gastrectomy
Lipase / metabolism
Male
Organ Size
Pancreas / anatomy & histology,  metabolism*
Proteins / metabolism
Rats
Stomach / physiology*
Trypsin / metabolism
Chemical
Reg. No./Substance:
0/Proteins; 9007-49-2/DNA; EC 3.1.1.3/Lipase; EC 3.2.1.-/Amylases; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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