Document Detail

Rat epidermal keratinocytes as an organotypic model for examining the role of Cx43 and Cx26 in skin differentiation.
MedLine Citation:
PMID:  16531317     Owner:  NLM     Status:  MEDLINE    
In order to characterize connexin expression and regulation in the epidermis, we have characterized a rat epidermal keratinocyte (REK) cell line that is phenotypically similar to basal keratinocytes in that they have the ability to differentiate into organotypic epidermis consisting of a basal cell layer, 2-3 suprabasal cell layers, and a cornified layer. RT-PCR revealed that REK cells express mRNA for Cx26, Cx31, Cx31.1, Cx37, and Cx43, which mimics the reported connexin profile for rat tissue. In addition, we report the expression of Cx30, Cx30.3, Cx40, and Cx45 in rat keratinocytes, highlighting the complexity of the connexin complement in rat epidermis. Furthermore, 3-dimensional analysis of organotypic skin revealed that Cx26 and Cx43 are exquisitely regulated during the differentiation process. The life-cycle of these connexins including their expression, transport, assembly into gap junctions, internalization, and degradation are elegantly depicted in organotypic epidermis as keratinocytes proceed from differentiation to programmed cell death.
Amy C Maher; Tamsin Thomas; Jessica L Riley; Greg Veitch; Qing Shao; Dale W Laird
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell communication & adhesion     Volume:  12     ISSN:  1541-9061     ISO Abbreviation:  Cell Commun. Adhes.     Publication Date:    2005 Jul-Dec
Date Detail:
Created Date:  2006-03-13     Completed Date:  2007-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101096596     Medline TA:  Cell Commun Adhes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-30     Citation Subset:  IM    
Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada.
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MeSH Terms
Cell Communication
Cell Differentiation*
Cell Line
Connexin 43 / genetics,  metabolism*
Connexins / genetics,  metabolism*
Gap Junctions / genetics,  metabolism
Gene Expression Regulation
Keratinocytes / cytology*,  metabolism
RNA, Messenger / metabolism
Skin / cytology*,  metabolism
Tissue Engineering
Reg. No./Substance:
0/Connexin 43; 0/Connexins; 0/RNA, Messenger; 127120-53-0/connexin 26

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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