Document Detail


Rat eosinophils stimulate the expansion of Cryptococcus neoformans-specific CD4(+) and CD8(+) T cells with a T-helper 1 profile.
MedLine Citation:
PMID:  21039463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experimental Cryptococcus neoformans infection in rats has been shown to have similarities with human cryptococcosis, revealing a strong granulomatous response and a low susceptibility to dissemination. Moreover, it has been shown that eosinophils are components of the inflammatory response to C. neoformans infections. In this in vitro study, we demonstrated that rat peritoneal eosinophils phagocytose opsonized live yeasts of C. neoformans, and that the phenomenon involves the engagement of FcγRII and CD18. Moreover, our results showed that the phagocytosis of opsonized C. neoformans triggers eosinophil activation, as indicated by (i) the up-regulation of major histocompatibility complex (MHC) class I, MHC class II and costimulatory molecules, and (ii) an increase in interleukin (IL)-12, tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) production. However, nitric oxide (NO) and hydrogen peroxide (H(2) O(2) ) synthesis by eosinophils was down-regulated after interaction with C. neoformans. Furthermore, this work demonstrated that CD4(+) and CD8(+) T lymphocytes isolated from spleens of infected rats and cultured with C. neoformans-pulsed eosinophils proliferate in an MHC class II- and class I-dependent manner, respectively, and produce important amounts of T-helper 1 (Th1) type cytokines, such as TNF-α and IFN-γ, in the absence of T-helper 2 (Th2) cytokine synthesis. In summary, the present study demonstrates that eosinophils act as fungal antigen-presenting cells and suggests that C. neoformans-loaded eosinophils might participate in the adaptive immune response.
Authors:
Ana P Garro; Laura S Chiapello; José L Baronetti; Diana T Masih
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-13
Journal Detail:
Title:  Immunology     Volume:  132     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-07     Completed Date:  2011-02-15     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  174-87     Citation Subset:  IM    
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Adaptive Immunity
Animals
Antigen Presentation
Antigen-Presenting Cells / immunology,  physiology
CD4-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / immunology*
Cryptococcosis / immunology*,  microbiology
Cryptococcus neoformans / immunology*
Eosinophils / immunology*,  physiology
Humans
Interferon-gamma / metabolism
Male
Phagocytosis
Rats
Rats, Wistar
Th1 Cells / immunology*,  metabolism
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma
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