| Rat cytomegalovirus induces cellular purine and pyrimidine nucleoside kinases in rat embryo fibroblasts and TK- rat-2 cells. Correlations with the antiviral activity of Acyclovir. | |
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MedLine Citation:
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PMID: 2982353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat cytomegalovirus (RCMV) induces a cytosol thymidine kinase (TK) in G0-phase rat embryo fibroblasts (REF), but not in a TK deficient rat cell line (R-2), though virus titers in both cell types reached comparable levels. The results indicate that TK is neither virus-coded nor is required for a productive infection in R-2 cells. A deoxycytidine kinase (dCK) is induced in either growing or RCMV-infected REF and R-2 cells, suggesting that dCK is essential for both host-cell and viral DNA synthesis. A deoxyguanosine kinase (dGK) is detectable in low concentrations in either growing or G0-phase REF and R-2 cells suggesting that this enzyme is cell-cycle independent. In contrast, RCMV induces high persisting levels of dGK, particularly in R-2 cells, indicating that this enzyme is of crucial importance for viral DNA synthesis. By comparison of thermostabilities and electrophoretic mobilities (Rf for TK, dCK and dGK were 0.12; 0.97; and 0.54, respectively) the enzymes were found to be substrate specific but of cellular origin. In contrast to TK and dCK, only dGK is inhibited by Acyclovir (Ki = 320 microM). It is suggested that RCMV inducable dGK is an important enzyme determining the in vitro anti-CMV activity of Acyclovir. |
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Authors:
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H Meijer; C A Bruggeman; P H Dormans; C P van Boven |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of virology Volume: 83 ISSN: 0304-8608 ISO Abbreviation: Arch. Virol. Publication Date: 1985 |
Date Detail:
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Created Date: 1985-03-14 Completed Date: 1985-03-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7506870 Medline TA: Arch Virol Country: AUSTRIA |
Other Details:
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Languages: eng Pagination: 181-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyclovir
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pharmacology* Animals Bromodeoxyuridine / pharmacology Cell Division Cells, Cultured Cytomegalovirus / drug effects, growth & development, physiology* Cytosol / enzymology Deoxycytidine Kinase / biosynthesis*, metabolism Electrophoresis, Polyacrylamide Gel Enzyme Induction Fibroblasts Interphase Phosphotransferases / antagonists & inhibitors, biosynthesis*, metabolism Phosphotransferases (Alcohol Group Acceptor)* Rats Substrate Specificity Thymidine Kinase / biosynthesis*, metabolism |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine; 59277-89-3/Acyclovir; EC 2.7.-/Phosphotransferases; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.113/deoxyguanosine kinase; EC 2.7.1.21/Thymidine Kinase; EC 2.7.1.74/Deoxycytidine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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