Document Detail


Rat costochondral chondrocytes produce 17beta-estradiol and regulate its production by 1alpha,25(OH)(2)D(3).
MedLine Citation:
PMID:  11792565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prior studies have shown that 17beta-estradiol (17beta-E(2)) regulates growth plate chondrocyte maturation and differentiation. This study examines the hypothesis that 17beta-E(2) is a local regulator of rat costochondral growth plate chondrocytes by determining whether these cells express aromatase mRNA and enzyme activity, produce 17beta-E(2), and regulate 17beta-E(2) production by vitamin D(3) metabolites in a gender-specific and cell-maturation-dependent manner. Aromatase gene expression was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and northern analysis of total RNA from male and female chondrocytes. Aromatase specific activity was measured in cell layer lysates of confluent male and female rat costochondral resting zone (RC) and growth zone (GC) cartilage cells that had been treated for 24 h with 1alpha, 25(OH)(2)D(3), 24R,25(OH)(2)D(3), or transforming growth factor (TGF)-beta1. 17beta-E(2) released into the culture media of treated cells was measured by radioimmunoassay (RIA). Female RC cells expressed the highest levels of aromatase mRNA compared with male RC cells and both male and female GC cells. Aromatase activity was present in male and female cells and was 1.6 times greater in female RC cells than female GC cells; male RC and GC cells displayed comparable levels. All cultures produced 17beta-E(2), with a 2.5-fold greater production by female RC cells than female GC cells or either cell type from male rats. Treatment of cultures with 1alpha,25(OH)(2)D(3) caused a dose-dependent increase in 17beta-E(2) production by female RC (1.5-fold greater than control cells) and female GC (threefold greater than control cells) cells. In contrast, 1alpha,25(OH)(2)D(3) had no effect on male GC cells and increased production in male RC cells by only 10% at the highest concentration of 1alpha,25(OH)(2)D(3) used. Neither 24R, 25(OH)(2)D(3) nor TGF-beta1 had an effect on 17beta -E(2) production. These results support our hypothesis and indicate that 17beta-E(2) is most likely a local regulator of rat costochondral growth plate chondrocytes.
Authors:
V L Sylvia; I Gay; R Hardin; D D Dean; B D Boyan; Z Schwartz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Bone     Volume:  30     ISSN:  8756-3282     ISO Abbreviation:  Bone     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-16     Completed Date:  2002-04-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8504048     Medline TA:  Bone     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-63     Citation Subset:  IM    
Affiliation:
Department of Orthopaedics, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
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MeSH Terms
Descriptor/Qualifier:
24,25-Dihydroxyvitamin D 3 / pharmacology
Animals
Aromatase / genetics,  metabolism
Calcitriol / pharmacology*
Cells, Cultured
Chondrocytes / drug effects*,  metabolism*
Estradiol / biosynthesis*
Female
Growth Plate / cytology,  drug effects,  metabolism
Male
RNA, Messenger / genetics,  metabolism
Rats
Sex Characteristics
Transforming Growth Factor beta / pharmacology
Grant Support
ID/Acronym/Agency:
DE-05937/DE/NIDCR NIH HHS; DE-08603/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Transforming Growth Factor beta; 32222-06-3/Calcitriol; 40013-87-4/24,25-Dihydroxyvitamin D 3; 50-28-2/Estradiol; EC 1.14.14.1/Aromatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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