| Rat corticosteroid binding globulin: primary structure and messenger ribonucleic acid levels in the liver under different physiological conditions. | |
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MedLine Citation:
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PMID: 2710140 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat corticosteroid binding globulin (CBG) cDNAs were isolated from a lambda gt11 liver cDNA library. When rat hepatic mRNA was hybrid selected and translated in vitro, a major product reacted with antibodies against rat CBG and its Mr (approximately 43,000) was consistent with a nonglycosylated, CBG precursor polypeptide. Two overlapping cDNAs produced a 1,432 nucleotide sequence with an open reading frame comprising 396 amino acids. This includes a potential signal peptide of 22 residues followed by the amino terminus of purified rat CBG. Rat CBG therefore contains 374 amino acids (Mr = 42,196), and has six consensus sites for N-glycosylation. There is 60% identity in the primary structures of rat and human CBG over 383 residues that comprise the human sequence. Furthermore, the single cysteine in rat CBG corresponds to one of two cysteines in human CBG, and this may be significant because a cysteine is located in the human CBG steroid binding site. Northern analysis of RNA from various rat tissues revealed an approximate 1.8 kilobase CBG mRNA only in the liver. Its relative abundance in a pregnant rat was only 30% higher than in an adult female; approximately 3-fold higher than in an adult male, and 25-fold higher than in the fetuses from the same animal. Southern analysis of rat genomic DNA suggests the presence of a single gene for CBG. |
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Authors:
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C L Smith; G L Hammond |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular endocrinology (Baltimore, Md.) Volume: 3 ISSN: 0888-8809 ISO Abbreviation: Mol. Endocrinol. Publication Date: 1989 Feb |
Date Detail:
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Created Date: 1989-06-07 Completed Date: 1989-06-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8801431 Medline TA: Mol Endocrinol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 420-6 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Western Ontario, Victoria Hospital, London, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Liver / analysis, physiology Molecular Sequence Data RNA, Messenger / analysis* Rats Transcortin / analysis*, blood, genetics |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9010-38-2/Transcortin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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