Document Detail

Rat allogeneic mesenchymal stem cells did not prolong the survival of corneal xenograft in a pig-to-rat model.
MedLine Citation:
PMID:  19891650     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Recent reports have demonstrated that mesenchymal stem cells (MSCs) can modulate/suppress immunologic responses through interactions with different immune cells. We performed this study in order to investigate the immunomodulatory effects of MSCs in corneal xenotransplantation. ANIMALS STUDIED: Pig and rat. PROCEDURES: We orthotopically transplanted pig corneas into rats and topically applied allogeneic rat MSCs to the corneas for 2 h immediately after transplantation. Graft survival was clinically assessed using slit-lamp biomicroscopy and the median survival time (MST) was calculated. The rejected grafts were histologically examined using antibodies against CD4, CD8, CD161, and CD68. The expression of IL-2, IL-6, IL-10, and IFN-gamma was also evaluated in the rejected grafts using an enzyme-linked immunosorbent assay. RESULTS: The survival of corneal xenografts was not significantly prolonged by MSC application (MST 10.5 days) compared with the controls (MST 9.67 days) (P = 0.4189). Histologically, the rejected grafts in both groups were massively infiltrated with neutrophils and macrophages. Some CD8+ T cells and rare NK cells were found in the rejected grafts. The levels of IL-6 and IL-10 were significantly increased in the rejected grafts from MSC-treated rats compared with the grafts from MSC-untreated rats. However, the levels of IL-2 and IFN-gamma were not different between the two groups. CONCLUSIONS: Topical application of allogeneic rat MSCs was ineffective in prolonging corneal xenograft survival in a pig-to-rat model.
Joo Youn Oh; Mee Kum Kim; Jung Hwa Ko; Hyun Ju Lee; Jin Hak Lee; Won Ryang Wee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary ophthalmology     Volume:  12 Suppl 1     ISSN:  1463-5224     ISO Abbreviation:  Vet Ophthalmol     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2009-11-06     Completed Date:  2010-02-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100887377     Medline TA:  Vet Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  35-40     Citation Subset:  IM    
Department of Ophthalmology, Seoul National University College of Medicine, 28 Yeongeon-dong, Chongno-gu, Seoul 110-744, Korea.
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MeSH Terms
Corneal Transplantation / veterinary*
Cytokines / genetics,  metabolism
Gene Expression Regulation / immunology
Graft Rejection / immunology*
Mesenchymal Stem Cells*
Rats, Sprague-Dawley
Specific Pathogen-Free Organisms
Transplantation, Heterologous / immunology*
Reg. No./Substance:

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